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saline Neg control, female | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
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Experiment Record Detail
Experiment
Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice
Experiment Condition
saline Neg control, female
Assay Description:
Fluorescent imaging for tdTomato+ cells
Tissue Measured:
kidney
Measurement Type:
Editing Efficiency
Record ID
15000001120
Editor
Delivery System
Delivery System Subtype
Guide
Vector
Model Species
Mouse
Model Name
B6.Cg-Gt(ROSA)26Sor^em1(CAG-tdTomato)JaHe
Application Method
intratracheal intubation
Application Site
lung airway epithelium
Dosage
Injection Frequency
once
Injection Rate
Injection Volume
40 microliters
Days post injection
4 weeks
Editor Format
Antidote Id
Antidote Description
Measured Values
Samples Size:
1
Replicate
Result (Editing Efficiency)
Measurement Units:
Signal
Mean
1 of 1 No red cells 
1
no red cells 
Experiment Wide Protocols
Title
Description
File Download
SCGE ID
Heaney_SATC Tissue Processing, Imaging and Analysis
Procedure for tissue preparation, imaging and analysis.
Heaney_SATC_Tissue_Processing_Imaging_and_Analysis.pdf
21000000025
Heaney-SATC_Gao-Validation_Intratracheal Delivery of AAV in Mice
Procedure for Intratracheal (IT) delivery of AAV in mouse lung.
Heaney-SATC_Gao-Validation_Intratracheal_Delivery_of_AAV_in_Mice.pdf
21000000023