Experiment Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice Experiment Condition saline Neg control, female Assay Description: PCR detection of AAV-delivered Cas9 and GFP by agarose gel electrophoresis Tissue Measured: lung Measurement Type: Delivery Efficiency Record ID 15000001139 Editor Delivery System Delivery System Subtype Guide Vector Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor^em1(CAG-tdTomato)JaHe Application Method intratracheal intubation Application Site lung airway epithelium Dosage Injection Frequency once Injection Rate Injection Volume 40 microliters Days post injection 4 weeks Editor Format Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Delivery Efficiency) Measurement Units: Signal Mean 3 of 3 Negative  1 Negative  2 Negative  3 Negative