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ExperimentIndependent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Experiment ConditionD10 only control, Female
Assay Description: Editing efficiency in small (<50000 um2) airways such as bronchioles and large (>50000 um2) airways such as the main bronchus, calculated by counting fluorescent cells (GFP) compared to DAPI stained cells.
Tissue Measured:epithelium of main bronchus
Cell Type :respiratory epithelial cell
Measurement Type:Editing Efficiency
Record ID15000001237

Editor
Delivery SystemD10
Delivery System SubtypeAmphiphilic peptide
Guide
Vector

Model SpeciesMouse
Model NameB6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J

Application Methodintranasal
Application Siteairway epithelium
Dosage40 micromolar peptide
Injection Frequencytwice, one day apart
Injection Rate
Injection Volume53 microliters
Days post injection1 week
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 2
 
Replicate Result (Editing Efficiency)
Measurement Units: % of cells
Mean 0.0 
1
2
  

Title Description File Download SCGE ID
Heaney-SATC_McCray-Validation_Lung Inflation and Fixation Protocol Procedure for mouse lung inflation and fixation. Heaney-SATC_McCray-Validation_Lung_Inflation_and_Fixation.pdf 21000000022
Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice Protocol Procedure for intranasal instillation of ribonucleoprotein/peptide complex in mice for delivery to the lung. Heaney-SATC_McCray-Validation_Intranasal_Instillation_in_Mice.pdf 21000000021
Heaney_SATC Tissue Processing, Imaging and Analysis Procedure for tissue preparation, imaging and analysis. Heaney_SATC_Tissue_Processing_Imaging_and_Analysis.pdf 21000000025