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Experiment: Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides

PI:   Jason D Heaney, PhD 
 

Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung airway epithilia via intranasal instillation into mTmG reporter mice. Editing is detected by production of GFP protein, and green fluorescence in airway linings
Editing Assay: - Editing efficiency in small (<50000 um2) airways such as bronchioles and large (>50000 um2) airways such as the main bronchus, calculated by counting fluorescent cells (GFP) compared to DAPI stained cells.

Organ System Overview


 
D10, RNP, Male
D10, RNP, Female
D237, RNP, Male
D237, RNP, Female
D10 only control, Male
D10 only control, Female
RNP only, Male
RNP only, Female
Saline Neg control, Male
Saline Neg control, Female
Legend:  
Delivery Efficiency
Editing Efficiency
Target Tissue
Not Available
 

 
Analyze Data Sets Available for this Experiment
Connective Tissue
  Brown adipose tissue (unspecified)
  Gonadal fat pad (unspecified)
  Subcutaneous adipose tissue (unspecified)
Reproductive
  Epididymis (unspecified)
  Testis (unspecified)
  Ovary (unspecified)
  Uterus (unspecified)
Renal/Urinary
  Kidney (unspecified)
Endocrine
  Pancreas (unspecified)
Haemolymphoid
  Spleen (unspecified)
Gastrointestinal
  Colon (unspecified)
  Duodenum (unspecified)
  Ileum (unspecified)
  Jejunum (unspecified)
  Stomach (unspecified)
Liver and Biliary
  Liver (unspecified)
Respiratory
  Epithelium of bronchiole (respiratory epithelial cell)  (TARGET)
  Epithelium of main bronchus (respiratory epithelial cell)  (TARGET)
Cardiovascular
  Heart (unspecified)
Musculoskeletal
  Diaphragm (unspecified)
  Extensor digitorum longus (unspecified)
  Gastrocnemius (unspecified)
  Soleus muscle (unspecified)
  Transversus abdominis muscle (unspecified)
Nervous
  Brain (unspecified)
Sensory
  Eye (unspecified)