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Validation Experiment: Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides

PI:   Jason D Heaney, PhD 

Description: Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung airway epithilia via intranasal instillation into mTmG reporter mice. Editing is detected by production of GFP protein, and green fluorescence in airway linings
Editing Assay: Editing efficiency in small (<50000 um2) airways such as bronchioles and large (>50000 um2) airways such as the main bronchus, calculated by counting fluorescent cells (GFP) compared to DAPI stained cells.
Download: Submitted files
Filters ..
  Tissues
Connective Tissue
  • Brown adipose tissue
  • Gonadal fat pad
  • Subcutaneous adipose tissue
Reproductive
  • Epididymis
  • Testis
  • Ovary
  • Uterus
Renal/Urinary
  • Kidney
Endocrine
  • Pancreas
Haemolymphoid
  • Spleen
Gastrointestinal
  • Colon
  • Duodenum
  • Ileum
  • Jejunum
  • Stomach
Liver and Biliary
  • Liver
Respiratory
  • Epithelium of bronchiole (respiratory epithelial cell)  (TARGET)
  • Epithelium of main bronchus (respiratory epithelial cell)  (TARGET)
Cardiovascular
  • Heart
Musculoskeletal
  • Diaphragm
  • Extensor digitorum longus
  • Gastrocnemius
  • Soleus muscle
  • Transversus abdominis muscle
Nervous
  • Brain
Sensory
  • Eye
Cell Types
 respiratory epithelial cell
Editors
 SpCas9
Guide Target Locus
 mTmG reporter transgene
Guides
 SpyCas9 g-loxP2_C9
  Delivery Systems
 D10
 D237
Models
 mTmG mouse (congenic)
  Sex
 Female
 Male
Select experimental variable to highlight records on the chart: 
 

Note: Hover over the bars to view additional information

Results
Independent_validation_for_McCray_Delivery_Team:_Delivery_of_CRISPR_Ribonucleoproteins_to_Airway_Epithelia_Using_Novel_Amphiphilic_Peptides
Editing Efficiency
Record Id Condition Tissue Cell Type Sex Editor Model Delivery Target Locus Guide Dosage Injection Frequency Editing Efficiency % of cells Editing Efficiency Signal Image
15000001140 D10, RNP, Male epithelium of bronchiole (TARGET) respiratory epithelial cell Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001141 D10, RNP, Male epithelium of main bronchus (TARGET) respiratory epithelial cell Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001142 D10, RNP, Male brown adipose tissue Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001143 D10, RNP, Male brain Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001144 D10, RNP, Male colon Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001145 D10, RNP, Male diaphragm Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001146 D10, RNP, Male duodenum Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001147 D10, RNP, Male extensor digitorum longus Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001148 D10, RNP, Male epididymis Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001149 D10, RNP, Male eye Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001150 D10, RNP, Male gastrocnemius Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001151 D10, RNP, Male heart Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001152 D10, RNP, Male ileum Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001153 D10, RNP, Male jejunum Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001154 D10, RNP, Male kidney Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001155 D10, RNP, Male liver Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001156 D10, RNP, Male pancreas Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001157 D10, RNP, Male gonadal fat pad Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001158 D10, RNP, Male subcutaneous adipose tissue Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001159 D10, RNP, Male soleus muscle Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001160 D10, RNP, Male spleen Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001161 D10, RNP, Male stomach Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001162 D10, RNP, Male transversus abdominis muscle Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001163 D10, RNP, Male testis Male SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001164 D10, RNP, Female epithelium of bronchiole (TARGET) respiratory epithelial cell Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001165 D10, RNP, Female epithelium of main bronchus (TARGET) respiratory epithelial cell Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001166 D10, RNP, Female brown adipose tissue Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001167 D10, RNP, Female brain Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001168 D10, RNP, Female colon Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001169 D10, RNP, Female diaphragm Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001170 D10, RNP, Female duodenum Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001171 D10, RNP, Female extensor digitorum longus Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001172 D10, RNP, Female eye Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001173 D10, RNP, Female gastrocnemius Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001174 D10, RNP, Female heart Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001175 D10, RNP, Female ileum Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001176 D10, RNP, Female jejunum Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001177 D10, RNP, Female kidney Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001178 D10, RNP, Female liver Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001179 D10, RNP, Female ovary Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001180 D10, RNP, Female pancreas Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001181 D10, RNP, Female gonadal fat pad Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001182 D10, RNP, Female subcutaneous adipose tissue Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001183 D10, RNP, Female soleus muscle Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001184 D10, RNP, Female spleen Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001185 D10, RNP, Female stomach Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001186 D10, RNP, Female transversus abdominis muscle Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001187 D10, RNP, Female uterus Female SpCas9 mTmG mouse (congenic) D10 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001188 D237, RNP, Male epithelium of bronchiole (TARGET) respiratory epithelial cell Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001189 D237, RNP, Male epithelium of main bronchus (TARGET) respiratory epithelial cell Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001190 D237, RNP, Male brown adipose tissue Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001191 D237, RNP, Male brain Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001192 D237, RNP, Male colon Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001193 D237, RNP, Male diaphragm Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001194 D237, RNP, Male duodenum Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001195 D237, RNP, Male extensor digitorum longus Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001196 D237, RNP, Male eye Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001197 D237, RNP, Male gastrocnemius Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001198 D237, RNP, Male heart Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001199 D237, RNP, Male ileum Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001200 D237, RNP, Male jejunum Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001201 D237, RNP, Male kidney Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001202 D237, RNP, Male liver Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001203 D237, RNP, Male pancreas Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001204 D237, RNP, Male gonadal fat pad Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001205 D237, RNP, Male subcutaneous adipose tissue Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001206 D237, RNP, Male soleus muscle Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001207 D237, RNP, Male spleen Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001208 D237, RNP, Male stomach Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001209 D237, RNP, Male transversus abdominis muscle Male SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001210 D237, RNP, Female epithelium of bronchiole (TARGET) respiratory epithelial cell Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001211 D237, RNP, Female epithelium of main bronchus (TARGET) respiratory epithelial cell Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001212 D237, RNP, Female brown adipose tissue Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001213 D237, RNP, Female brain Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001214 D237, RNP, Female colon Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001215 D237, RNP, Female diaphragm Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001216 D237, RNP, Female duodenum Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001217 D237, RNP, Female extensor digitorum longus Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001218 D237, RNP, Female eye Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001219 D237, RNP, Female gastrocnemius Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001220 D237, RNP, Female heart Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001221 D237, RNP, Female ileum Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001222 D237, RNP, Female jejunum Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001223 D237, RNP, Female kidney Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001224 D237, RNP, Female liver Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001225 D237, RNP, Female ovary Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001226 D237, RNP, Female pancreas Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001227 D237, RNP, Female gonadal fat pad Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001228 D237, RNP, Female subcutaneous adipose tissue Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001229 D237, RNP, Female soleus muscle Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001230 D237, RNP, Female spleen Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001231 D237, RNP, Female stomach Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001232 D237, RNP, Female transversus abdominis muscle Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001233 D237, RNP, Female uterus Female SpCas9 mTmG mouse (congenic) D237 mTmG reporter transgene SpyCas9 g-loxP2_C9 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001234 D10 only control, Male epithelium of bronchiole (TARGET) respiratory epithelial cell Male mTmG mouse (congenic) D10 40 micromolar peptide twice, one day apart
15000001235 D10 only control, Male epithelium of main bronchus (TARGET) respiratory epithelial cell Male mTmG mouse (congenic) D10 40 micromolar peptide twice, one day apart
15000001236 D10 only control, Female epithelium of bronchiole (TARGET) respiratory epithelial cell Female mTmG mouse (congenic) D10 40 micromolar peptide twice, one day apart
15000001237 D10 only control, Female epithelium of main bronchus (TARGET) respiratory epithelial cell Female mTmG mouse (congenic) D10 40 micromolar peptide twice, one day apart
15000001238 RNP only, Male epithelium of bronchiole (TARGET) respiratory epithelial cell Male SpCas9 mTmG mouse (congenic) mTmG reporter transgene SpyCas9 g-loxP2_C9 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001239 RNP only, Male epithelium of main bronchus (TARGET) respiratory epithelial cell Male SpCas9 mTmG mouse (congenic) mTmG reporter transgene SpyCas9 g-loxP2_C9 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001240 RNP only, Female epithelium of bronchiole (TARGET) respiratory epithelial cell Female SpCas9 mTmG mouse (congenic) mTmG reporter transgene SpyCas9 g-loxP2_C9 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001241 RNP only, Female epithelium of main bronchus (TARGET) respiratory epithelial cell Female SpCas9 mTmG mouse (congenic) mTmG reporter transgene SpyCas9 g-loxP2_C9 2.5 micromolar SpCas9, 2 micromolar RNA twice, one day apart
15000001242 Saline Neg control, Male epithelium of bronchiole (TARGET) respiratory epithelial cell Male mTmG mouse (congenic) saline twice, one day apart
15000001243 Saline Neg control, Male epithelium of main bronchus (TARGET) respiratory epithelial cell Male mTmG mouse (congenic) saline twice, one day apart
15000001244 Saline Neg control, Female epithelium of bronchiole (TARGET) respiratory epithelial cell Female mTmG mouse (congenic) saline twice, one day apart
15000001245 Saline Neg control, Female epithelium of main bronchus (TARGET) respiratory epithelial cell Female mTmG mouse (congenic) saline twice, one day apart

Experiment Wide Protocols

Title Description File Download SCGE ID
Heaney-SATC_McCray-Validation_Lung Inflation and Fixation Protocol Procedure for mouse lung inflation and fixation. Heaney-SATC_McCray-Validation_Lung_Inflation_and_Fixation.pdf 21000000022
Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice Protocol Procedure for intranasal instillation of ribonucleoprotein/peptide complex in mice for delivery to the lung. Heaney-SATC_McCray-Validation_Intranasal_Instillation_in_Mice.pdf 21000000021
Heaney_SATC Tissue Processing, Imaging and Analysis Procedure for tissue preparation, imaging and analysis. Heaney_SATC_Tissue_Processing_Imaging_and_Analysis.pdf 21000000025