Experiment: Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
PI: Jason D Heaney, PhD| Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung airway epithilia via intranasal instillation into mTmG reporter mice. Editing is detected by production of GFP protein, and green fluorescence in airway linings |
| Editing Assay: |
- Editing efficiency in small (<50000 um2) airways such as bronchioles and large (>50000 um2) airways such as the main bronchus, calculated by counting fluorescent cells (GFP) compared to DAPI stained cells. |
Organ System Overview