Validation Experiment: Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
PI: Jason D Heaney, PhD
Description: Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung airway epithilia via intranasal instillation into mTmG reporter mice. Editing is detected by production of GFP protein, and green fluorescence in airway linings
Editing Assay:
Editing efficiency in small (<50000 um2) airways such as bronchioles and large (>50000 um2) airways such as the main bronchus, calculated by counting fluorescent cells (GFP) compared to DAPI stained cells.
Parent Project: BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - McCray Validation (McCray Jr., Paul)
Download: Submitted files
Original Experiment/s that are being validated:
Analyze Data Sets Available for this Experiment
Connective Tissue
| Brown adipose tissue |
| Gonadal fat pad |
| Subcutaneous adipose tissue |
| Epididymis |
| Testis |
| Ovary |
| Uterus |
| Kidney |
| Pancreas |
| Spleen |
| Colon |
| Duodenum |
| Ileum |
| Jejunum |
| Stomach |
| Liver |
| Epithelium of bronchiole (respiratory epithelial cell) (TARGET) |
| Epithelium of main bronchus (respiratory epithelial cell) (TARGET) |
| Heart |
| Diaphragm |
| Extensor digitorum longus |
| Gastrocnemius |
| Soleus muscle |
| Transversus abdominis muscle |
| Brain |
| Eye |
Organ System Overview
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