mTmG mouse (congenic) | SCGE Toolkit

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Summary Projects & Experiments

SCGE ID	13000000036
Name	mTmG mouse (congenic)
Official Name	B6.129(Cg)-Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/J
Alias	mTmG; mT/mG; CAG-lox-tdTomato-lox-GFP
Species	Mouse
Type	Animal
Description	mTmG is a double-fluorescent reporter transgenic mouse which expresses membrane-targeted tdTomato flanked by loxP sequences, followed by membrane-targeted GFP. After genomic cleavage by Cas9 at two sites, or Cre recombinase between loxP sites, tdTomato expression is lost and GFP is expressed.

Source	The Jackson Laboratory
RRID	RRID:IMSR_JAX:007676

Transgene Description	mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated deletion of the ORF. after genomic cleavage by Cas9 at 2 sites flanking tdTomato, the membrane-targeted GFP is expressed.
Reporter	tdTomato/GFP double reporter
Annotated Map	mTmG plasmid

This Model System: mTmG mouse (congenic) is being used ...

Project	Initiative	Contact PI
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Experiments Testing preparation for independent validation at The Jackson Laboratory Small Animal Testing Center Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain	Delivery Systems	Erik J Sontheimer, PhD (University of Massachusetts Medical School, Worcester)
BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice Experiments Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides	Animal Reporter and Testing Center	Jason D Heaney, PhD (Baylor College of Medicine)
The Jackson Laboratory Gene Editing Testing Center (JAX-GETC) Experiments Independent validation of Sontheimer delivery platform using heavily modified guide RNAs complexed with Cas9 proteins to deliver CRISPR/Cas9 to mouse brain	Animal Reporter and Testing Center	Stephen A Murray, PhD (Jackson Laboratory)