Experiment Independent validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain Experiment Condition Arm 2b: BI28 Astrocyte Specific Promoter, Positive Delivery Control Animals, Females Assay Description: Identification of of GFP-positive nuclei (green) Tissue Measured: brain Cell Type : astrocyte Measurement Type: Delivery Efficiency Record ID 15000001461 Editor SaCas9 Delivery System Delivery System Subtype Guide Vector BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method Application Site brain Dosage 0.25E+11 viral genomes/gram for each virus Injection Frequency once Injection Rate Injection Volume 5 microliters/gram Days post injection 4 weeks Editor Format ssDNA, packaged in two AAVs with astrocyte-specific promoter Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Delivery Efficiency) Measurement Units: Signal Mean 3 of 3 present  1 Present  2 Present  3 Present