BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2

SCGE ID	14000000039
Name	BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2
Description	Novel engineered AAV BI28 variant expressing NLS-GFP driven by the glial fibrillary acidic protein (GFAP) promoter and dual sgRNAs with modified scaffolds
Type	Viral Vector
Subtype	AAV

Source	Deverman Lab

Genome Serotype	AAV2
Capsid Serotype	AAV BI28 (novel engineered variant)
Capsid Variant	BI28: 7-mer peptide insertion (VP1 588-589) within AAV9 K449R
Stock/Catalog/RRID	BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2

Titer Method	ddPCR, average of two titers using ITR and WPRE primers

This Vector/Format: BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2 is being used ...

Project	Initiative	Contact PI
Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System Experiments Testing gRNA sequence and gRNA scaffold modified in Ai9 mice.	Delivery Systems Initiative	Benjamin E Deverman, PhD (Broad Institute)
BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice Experiments Independent validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain	Animal Reporter and Testing Center Initiative	Jason D Heaney, PhD (Baylor College of Medicine)

Vector/Format: BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2