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Editors
 Alt-R® S.p. Cas9 Nuclease V3
Guide Target Locus
 Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn
  Guides
 R26-1
 R26-2
 R26-3
 R26-52
 R26-59
 R26-69
Models
 TLR-2 mouse heterozygous blastocyst
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Results

Validating_Traffic_Light_Reporter_2_(TLR2)_Mouse_Model_in_Heterozygous_Blastocysts_using_SpCas9
Editing Efficiency
Record Id Condition Editor Model Target Locus Guide Editing Efficiency Signal Editing Efficiency % of blasts with RFP activation Editing Efficiency % of blasts with edits Image
15000003450 Guide 26-1 Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse heterozygous blastocyst Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-1
15000003451 Guide 26-2 Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse heterozygous blastocyst Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2
15000003452 Guide 26-3 Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse heterozygous blastocyst Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-3
15000003453 Guide 26-52 Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse heterozygous blastocyst Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52
15000003454 Guide 26-59 Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse heterozygous blastocyst Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-59
15000003455 Guide 26-69 Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse heterozygous blastocyst Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-69