Validating Traffic Light Reporter 2 (TLR2) Mouse Model in Heterozygous Blastocysts using SpCas9 |
Guide 26-52 |
Reporter activation by NHEJ is calculated as the percent of blasts that express tagRFP out of the total blasts imaged. Approximately one third of NHEJ events are detected. |
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Editing Efficiency |
15000003453 |
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Alt-R® S.p. Cas9 Nuclease V3 |
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R26-52 |
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Mouse |
TLR-2 Heterozygous Blastocyst |
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Electroporation |
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96 hours |
RNP |
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Measured Values |
Samples Size: | 0 |
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Mean |
53  |
1 |
53.0  |
Mean |
83  |
1 |
83.0  |
Mean |
# blasts analyzed  |
1 |
102 blasts analyzed for RFP  |
Mean |
# blasts analyzed  |
1 |
12 blasts anlayzed for edits  |