Experiment Validating Traffic Light Reporter 2 (TLR2) Mouse Model in Homozygous Blastocysts using SpCas9 Experiment Condition Guide 26-1 Assay Description: Reporter activation by NHEJ is calculated as the percent of blasts that express tagRFP out of the total blasts imaged. Approximately one third of NHEJ events are detected. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000003456 Editor Alt-R® S.p. Cas9 Nuclease V3 Delivery System Delivery System Subtype Guide R26-1 Vector Model Species Mouse Model Name TLR-2 homozygous blastocyst Application Method Electroporation Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection 96 hours Editor Format RNP Antidote Id Antidote Description Measured Values Samples Size: 0   Replicate Result (Editing Efficiency) Measurement Units: % of blasts with RFP activation Mean 11.8  1 11.8  Measurement Units: % of blasts with edits Mean 84.52  1 84.52  Measurement Units: Signal Mean # blasts analyzed  1 170 blasts analyzed for RFP  Mean # blasts analyzed  1 84 blasts analyzed for edits