SCGE ID11000000118
SymbolAlt-R® S.p. Cas9 Nuclease V3
DescriptionRecombinant S. pyogenes Cas9 nuclease, purified from an E. coli strain expressing the nuclease. Contains nuclear localization sequence (NLS) and C-terminal 6-His tag. Provided in solution at 10 µg/µL. 100 µg of Cas9 nuclease = 610 pmol.
SpeciesStreptococcus pyogenes
TypeCas Nuclease
SubtypeCas9

ActivityDouble strand cleavage
Substratedouble stranded DNA
DSB Cleavage TypeBlunt
PAM3' NGG
FusionNLS

SourceIntegrated DNA Technologies


Publication Title
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI

This Genome Editor: Alt-R® S.p. Cas9 Nuclease V3 is being used ...
Project Initiative Contact PI
Delivery Systems Paul B McCray, Jr, MD
(University of Iowa)
NIH Report
Animal Reporter and Testing Center Stephen A Murray, PhD
(Jackson Laboratory)
NIH Report