Validating Traffic Light Reporter 2 (TLR2) Mouse Model in Homozygous Blastocysts using SpCas9
Experiment Condition
Guide 26-2
Assay Description:
Reporter activation by NHEJ is calculated as the percent of blasts that express tagRFP out of the total blasts imaged. Approximately one third of NHEJ events are detected.
Validation of Traffic Light Reporter 2 (TLR2) Mouse Model in Homozygote Blastocysts
Zygotes were electroporated with Cas9 RNP along with guides and cultured 96 hours to blastocyst stage. Blastocysts were imaged for tagRFP fluorescence and scored for fluorescence signal.