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Editors
 Alt-R® S.p. Cas9 Nuclease V3
Guide Target Locus
 Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn
  Guides
 R26-1
 R26-2
 R26-3
Models
 TLR-2 mouse homozygous blastocyst
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Results

Validating_Traffic_Light_Reporter_2_(TLR2)_Mouse_Model_in_Homozygous_Blastocysts_using_SpCas9
Editing Efficiency
Record Id Condition Editor Model Target Locus Guide Editing Efficiency Signal Editing Efficiency % of blasts with RFP activation Editing Efficiency % of blasts with edits Image
15000003456 Guide 26-1 Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse homozygous blastocyst Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-1
15000003457 Guide 26-2 Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse homozygous blastocyst Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2
15000003458 Guide 26-3 Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse homozygous blastocyst Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-3

Validation of Traffic Light Reporter 2 (TLR2) Mouse Model in Homozygote Blastocysts

Zygotes were electroporated with Cas9 RNP along with guides and cultured 96 hours to blastocyst stage. Blastocysts were imaged for tagRFP fluorescence and scored for fluorescence signal.