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ExperimentAAV tropism in kidney organoids cultured under flow
Experiment ConditionAAV2/8, No Flow on Chip
Assay Description: Calculated total transduction efficiency by counting fluorescent cells (GFP) compared to DAPI stained cells.
Tissue Measured:
Measurement Type:Delivery Efficiency
Record ID15000003409

Editor
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAV2/8CMVeGFP;

Model SpeciesHuman
Model NameHuman kidney organoid (stem cell-derived)

Application Methodvirus
Application Site
DosageMOI=10^5
Injection Frequency
Injection Rate
Injection Volume
Days post injection7 days
Editor FormatAAV vector encoded
Antidote Id
Antidote Description

Antibody RRIDRRID:AB_300798
Antibody Other IDab13970 (Abcam)
Antibody DescriptionChicken anti-GFP polyclonal antibody

Measured Values
Samples Size: 2
 
Replicate Result (Delivery Efficiency)
Measurement Units: %GFP+ Area
Mean
1 0.00037 
2