Experiment AAV tropism in kidney organoids cultured under flow Experiment Condition AAV2/2, Low Flow on Chip Assay Description: Calculated total transduction efficiency by counting fluorescent cells (GFP) compared to DAPI stained cells. Tissue Measured: Measurement Type: Delivery Efficiency Record ID 15000003412 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV2/2CMVeGFP; Model Species Human Model Name Human kidney organoid (stem cell-derived) Sample Age 33-44 Application Method virus Application Site Dosage MOI=10^5 Injection Frequency Injection Rate Injection Volume Days post injection 7 days Editor Format AAV vector encoded Antidote Id Antidote Description Antibody RRID RRID:AB_300798 Antibody Other ID ab13970 (Abcam) Antibody Description Chicken anti-GFP polyclonal antibody Antibody RRID RRID:AB_2336558 Antibody Other ID B-1325-2 (Vector Labs) Antibody Description Lotus tetragonolobus lectin (LTL) Antibody RRID RRID:AB_354920 Antibody Other ID AF1658 (R&D Systems) Antibody Description Goat anti-podocalyxin polyclonal antibody Measured Values Samples Size: 2   Replicate Result (Delivery Efficiency) Measurement Units: %GFP+ Area Mean 3.57  1 0.931  2 6.204  Measurement Units: %GFP+ LTL+ / LTL+ cells Mean 3.85  1 3.849576  Measurement Units: %GFP+ PODXL+ / PODXL+ cells Mean 0.97  1 0.970969