Experiment AAV tropism in kidney organoids cultured under flow Experiment Condition AAV2/8, Low Flow on Chip Assay Description: Calculated total transduction efficiency by counting fluorescent cells (GFP) compared to DAPI stained cells. Tissue Measured: Measurement Type: Delivery Efficiency Record ID 15000003413 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV2/8CMVeGFP; Model Species Human Model Name Human kidney organoid (stem cell-derived) Application Method virus Application Site Dosage MOI=10^5 Injection Frequency Injection Rate Injection Volume Days post injection 7 days Editor Format AAV vector encoded Antidote Id Antidote Description Antibody RRID RRID:AB_300798 Antibody Other ID ab13970 (Abcam) Antibody Description Chicken anti-GFP polyclonal antibody Measured Values Samples Size: 2   Replicate Result (Delivery Efficiency) Measurement Units: %GFP+ Area Mean 0.85  1 1.661  2 0.044