Experiment Biomarker assays to evaluate toxicity and inflammation induced by AAVs in ES cell derived kidney organoids. Experiment Condition AAV2/2 Assay Description: KIM-1 and MCP-1 levels were measured using the Meso Scale Discovery (MSD) platform Tissue Measured: Measurement Type: Biomarker Detection Record ID 15000003425 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV2/2CMVeGFP; Model Species Human Model Name Human kidney organoid (ESC-derived) Sample Age 33-44 Sample Sex Female Application Method virus Application Site Dosage MOI=10^5 Injection Frequency Injection Rate Injection Volume Days post injection 7 days Editor Format AAV vector encoded Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Biomarker Detection) Measurement Units: KIM-1 (pg/mL) Mean 1.69  1 3.52  2 0  3 1.55  Measurement Units: MCP-1 (pg/mL) Mean 1319.29  1 1668.54  2 890.45  3 1398.88