Experiment Evaluation of DNA damage, cellular toxicity and inflammatory markers following saCas9 and gRNAs delivery by AAV2 in kidney organoids. Experiment Condition no AAV control Assay Description: Compartmental DNA damage was calculated as the percent of YH2AX co-staining among LTL, CHD1 and MEIS1/2/3 positive cells Tissue Measured: Measurement Type: Biomarker Detection Record ID 15000003441 Editor Delivery System Delivery System Subtype Guide Vector Model Species Human Model Name Human kidney organoid (stem cell-derived) Application Method no virus control Application Site Dosage MOI=10^5 Injection Frequency Injection Rate Injection Volume Days post injection 7 days Editor Format no editor Antidote Id Antidote Description Antibody RRID RRID:AB_2750570 Antibody Other ID Active Motif Cat# 39795 Antibody Description Mouse anti-meis1/2/3 Antibody RRID RRID:AB_2118010 Antibody Other ID Cell Signaling Technology Cat# 2577 Antibody Description Rabbit anti-Phospho-Histone H2A.X (Ser139) Antibody RRID RRID:AB_307284 Antibody Other ID Abcam Cat# ab9498 Antibody Description Mouse anti-CD31 antibody Antibody RRID RRID:AB_2336558 Antibody Other ID Vector Laboratories Cat# B-1325 Antibody Description Lotus tetragonolobus lectin (LTL) Antibody RRID RRID:AB_2116561 Antibody Other ID R and D Systems Cat# AF1750 Antibody Description Goat anti-KIM1/HAVCR Measured Values Samples Size: 3   Replicate Result (Biomarker Detection) Measurement Units: %KIM1+/LTL+ cells Mean 15.02  1 25  2 16.66667  3 0  4 14.2857  5 20  6 16.666667  7 12.5  Measurement Units: %YH2AX+/CHD1+ cells Mean 0.6  1 0.78125  2 1.02040816326531  3 0  Measurement Units: %YH2AX+/LTL+ cells Mean 0.71  1 0.218818380743982  2 1.07962213225371  3 0.845070422535211  Measurement Units: %YH2AX+/MEIS1/2/3+ cells Mean 2.99  1 4.28134556574923  2 1.98757763975155  3 2.71565495207668  Measurement Units: IL-10 (pg/mL) Mean 455.89  1 446.542860760008  2 455.804285816509  3 482.350513357372  4 428.17839303964  5 468.837814509775  6 457.181289899693  7 482.46822882869  8 425.77992425901  Measurement Units: IL-6 (pg/mL) Mean 2.57  1 1.65477687183361  2 1.71337051363705  3 3.94681048062236  4 2.42342203553629  5 2.45673546508562  6 3.09580748201716  7 3.14160853809432  8 2.16536385889831  Measurement Units: IL1-beta (pg/mL) Mean 1.9  1 0.989206294342492  2 1.37804389357951  3 2.63693681324954  4 2.4051573924998  5 2.20678733880701  6 1.16206361649702  7 2.68628458619969  8 1.7561532152817  Measurement Units: KIM-1 (pg/mL) Mean 48.74  1 51.3995733584671  2 43.6964290781139  3 66.0926756135368  4 50.1231942837828  5 38.8285557898405  6 46.6445392989742  7 46.4596779579597  8 46.6990608708682  Measurement Units: MCP-1 (pg/mL) Mean 6.81  1 5.0763176199031  2 6.09109175920988  3 10.7828875743226  4 7.80897357375238  5 5.31558434785989  6 6.13127470317533  7 8.15865512382412  8 5.08765217318633  Measurement Units: NGAL (pg/mL) Mean 2339.41  1 1945.87177714803  2 2378.00592659228  3 2450.71409648479  4 2402.33372573432  5 1891.05748937221  6 2587.61534903933  7 2628.15126475537  8 2431.52943287365