Experiment Evaluation of DNA damage, cellular toxicity and inflammatory markers following saCas9 and gRNAs delivery by AAV2 in kidney organoids. Experiment Condition AAV2/2-GFP Assay Description: Compartmental DNA damage was calculated as the percent of YH2AX co-staining among LTL, CHD1 and MEIS1/2/3 positive cells Tissue Measured: Measurement Type: Biomarker Detection Record ID 15000003442 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector Model Species Human Model Name Human kidney organoid (stem cell-derived) Application Method virus Application Site Dosage MOI=10^5 Injection Frequency Injection Rate Injection Volume Days post injection 7 days Editor Format no editor Antidote Id Antidote Description Antibody RRID RRID:AB_2750570 Antibody Other ID Active Motif Cat# 39795 Antibody Description Mouse anti-meis1/2/3 Antibody RRID RRID:AB_2118010 Antibody Other ID Cell Signaling Technology Cat# 2577 Antibody Description Rabbit anti-Phospho-Histone H2A.X (Ser139) Antibody RRID RRID:AB_307284 Antibody Other ID Abcam Cat# ab9498 Antibody Description Mouse anti-CD31 antibody Antibody RRID RRID:AB_2336558 Antibody Other ID Vector Laboratories Cat# B-1325 Antibody Description Lotus tetragonolobus lectin (LTL) Antibody RRID RRID:AB_2116561 Antibody Other ID R and D Systems Cat# AF1750 Antibody Description Goat anti-KIM1/HAVCR Measured Values Samples Size: 3   Replicate Result (Biomarker Detection) Measurement Units: %KIM1+/LTL+ cells Mean 62.94  1 50  2 66.66667  3 60  4 80  5 71.43  6 62.5  7 50  Measurement Units: %YH2AX+/CHD1+ cells Mean 24.04  1 15.7534246575342  2 29.8013245033113  3 26.5625  Measurement Units: %YH2AX+/LTL+ cells Mean 58.17  1 64.3356643356643  2 57.5418994413408  3 52.6315789473684  Measurement Units: %YH2AX+/MEIS1/2/3+ cells Mean 30.61  1 23.3995584988962  2 32.9918032786885  3 35.4285714285714  Measurement Units: IL-10 (pg/mL) Mean 491.34  1 512.276847412247  2 490.682364581603  3 513.103953627712  4 450.859966622963  5 515.578696906728  6 479.309837807072  7 511.275559120074  8 457.67154745967  Measurement Units: IL-6 (pg/mL) Mean 7.91  1 7.72452780216676  2 8.8784773684702  3 5.51973586207241  4 10.1474367515684  5 7.97776945388018  6 7.4266544119219  7 7.43532003372835  8 8.15770843055003  Measurement Units: IL1-beta (pg/mL) Mean 4.34  1 4.33968406632017  2 4.80608939258317  3 3.74139531934747  4 3.98690556903495  5 4.89736567174163  6 4.55175310293446  7 3.77368074815052  8 4.6430370516758  Measurement Units: KIM-1 (pg/mL) Mean 72  1 71.5833165541194  2 90.2160638029641  3 59.3236870646692  4 81.469906438934  5 78.8138728778462  6 63.8860238886115  7 61.428165238845  8 69.2591122635337  Measurement Units: MCP-1 (pg/mL) Mean 16  1 18.0070916190554  2 16.1686378082723  3 8.70221005688366  4 17.4432793219612  5 32.4877578217399  6 9.74665350939028  7 16.4090371835311  8 9.0209742116227  Measurement Units: NGAL (pg/mL) Mean 3303.99  1 3461.44354915398  2 4007.01594029684  3 2383.42759214056  4 2606.11627788187  5 3466.97334025547  6 2910.32739554959  7 4761.98444276883  8 2834.6371167695