Validating Traffic Light Reporter 7 (TLR7) Mouse Model for HDR in Heterozygous Blastocysts
Experiment Condition
RNP & ssODN
Assay Description:
Reporter activation by NHEJ is calculated as the percent of blasts that express Katushka2S (RFP) out of the total blasts imaged. Approximately one third of NHEJ events are detected.
HDR Validation of Traffic Light Reporter 7 (TLR7) Mouse Model in Heterozygous Blastocysts
Zygotes were electroporated with Cas9 RNP along with guides and HDR donor, and cultured 96 hours to blastocyst stage. Blastocysts were imaged for Venus and Katushka2S (RFP) fluorescence and scored for fluorescence signal.