Development System
SgaCas9-sgRNA1-NT | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
    
    
    
    
    
        
        
        
        
            
                
                    
                        
                        
                        
                        
                        
                        
                            
                        
                    
                        
                        
                            
                            
                            
                        
    
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            | Genome editing activity of four Streptococcus Cas9s by gRNA targeting the HBE promoter in HEK293T cells. |  | SgaCas9-sgRNA1-NT |  
                | Calculated editing efficiency by NGS sequencing at target site. |  
                |  |  
                | Editing Efficiency |  | 15000005341 |  | 
 |  | S. gallolyticus Cas9 |  | Lipofectamine 3000 |  | Commercial Reagent |  | NT-sgRNA |  |  |  | 
 |  | Human |  | HEK-293T |  | 
 |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | 
 |  
                | Measured Values |  
                | Samples Size: | 3 |  |  |  
                
                
             
                 
                     
                     
                      
                
                | Mean | 0.14  |  
                
                | 1 | 0.150472384617282  |  
                
                | 2 | 0.140721196130167  |  
                
                | 3 | 0.114201986683617  |  |  |  |