Experiment Genome editing activity of four Streptococcus Cas9s by gRNA targeting the HBE promoter in HEK293T cells. Experiment Condition SpyCas9-sgRNA1 Assay Description: Calculated editing efficiency by NGS sequencing at target site. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000005356 Editor S. pyogenes Cas9 Delivery System Lipofectamine 3000 Delivery System Subtype Commercial Reagent Guide SpyCas9-sgRNA1 Vector Model Species Human Model Name HEK-293T Application Method Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection Editor Format Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % of reads Mean 49.43  1 49.8851540616246  2 50.7256074781702  3 47.6731768670353