Project: Cas9 ribonucleoprotein delivery targeted to kidney epithelium
PI: Benjamin S Freedman, PhD> Ross C. Wilson, PhD>
Submissions Details
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| Experiment Name | Type | Description |
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Podocyte-specific gene editing in human kidney organoids
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In Vitro | Kidney organoids were derived from a human iPS cell line with Ai9 (tdTomato) fluorescence-on reporter knocked into the AAVS1 safe harbor locus. Intact kidney organoids were transfected with CRISPR ribonucleoprotein complexes with and without molecular targeting agent (MTA) specific for podocytes. Genome editing events were detected by induction of tdTomato from the Ai9 reporter. |