Summary

SCGE ID10000000212
Lab IDSTS135 (PCSK9c; Sp_t2:Sp_c20_PCSK9c)
Sourcein house production
Target LocusPcsk9
Speciesmouse
DescriptionTargets endogenous mouse locus
Guide CompatibilitySpyCas9
Target SequenceGCTCGCCCTCCCGTCCCAGG
Target Sequence + PAMGCTCGCCCTCCCGTCCCAGGAGG
PositionGRCm39/mm39/chr4:106299531-106321522
Full Guide Sequence

Sp_t2: AGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUU

Sp_c20_PCSK9c: GCUCGCCCUCCCGUCCCAGGGUUUUAGAGCUAUGCU

Spacer SequenceGCUCGCCCUCCCGUCCCAGG
Spacer Length20
Modifications

Sp_t2: (mA)#(mG)#(mC)#(mA)(mU)(mA)(mG)(mC)(mA)(mA)(mG)(rU)(rU)(mA)(rA)(mA)(mA)(rU)(mA)(mA)(mG)(mG)(rC)(rU)(mA)(rG)(rU)(rC)(mC)(rG)(rU)(rU)(mA)(mU)(mC)(mA)(mA)(mC)(mU)(mU)(mG)(mA)(mA)(mA)(mA)(mA)(mG)(mU)(mG)(mG)(mC)(mA)(mC)(mC)(mG)(mA)(mG)(mU)(mC)(mG)(mG)(mU)(mG)(mC)#(mU)#(mU)#(mU)

Sp_c20_PCSK9c: (mG)#(mC)#(mU)#(mC)(mG)(mC)(mC)(mC)(mU)(mC)(fC)(fC)(fG)(fU)(rC)#(rC)#(fC)(fA)(rG)#(mG)(mG)(rU)#(rU)#(rU)#(fU)(fA)(mG)(mA)(mG)(mC)(mU)(mA)(mU)#(mG)#(mC)#(mU)

Repeat SequenceGUUUUAGAGCUAUGCU
Anit-Repeat SequenceAGCAUAGCAAGUUAAAAU
Stemloop 1 SequenceAAGGCUAGUCCGU
Stemloop 2 SequenceAACUUGAAAAAGUG
Stemloop 3 SequenceGCACCGAGUCGGUGC

Related Editors

  • SpyCas9-3xNLS

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    Projects & Experiments

    This Guide: STS135 (PCSK9c; Sp_t2:Sp_c20_PCSK9c) is being used ...
    Project Initiative Contact PI
    Delivery Systems Erik J Sontheimer, PhD
    (University of Massachusetts Medical School, Worcester)    		 NIH Report