Negative control gRNA | SCGE Toolkit

SCGE ID	10000000369
Lab ID	Negative control gRNA
Source	IDT
Species	mouse
Description	Negative control guide RNA avoid any targets in human/mouse/rat
Guide Compatibility	SpyCas9

Full Guide Sequence	tracRNA:AGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUU; crRNA:CGUUAAUCGCGUAUAAUACG
Spacer Sequence	CGUUAAUCGCGUAUAAUACG
Spacer Length	20
Modifications	5' AND 3' TERMINALS 2'-O-METHYL ANALOGS AND 3’-PHOSPHOROTHIOATE INTERNUCLEOTIDE LINKAGES, ATTO 550 MODIFICATION ON 5' END OF TRACRRNA.

This Guide: Negative control gRNA is being used ...

Project	Initiative	Contact PI
Comparing the efficiency and cellular impact of non-viral, hepatocyte-targeted delivery vehicles with viral-mediated gene delivery in a microphysiological liver on chip platform Experiments Testing different ratios of Lipofectamine-RNPs after 24 hours for determination of positive control Imaging quantification of transfection efficiency with varying dosages of nanoparticles encapsulated with Cas9/sgRNA RNP on the liver-on-chip model system	Collaborative Opportunity Fund	Ross C. Wilson, PhD Samira Kiani, PhD Shaoqin (Sarah) Gong, PhD (University of Pittsburgh)

Guide: Negative control gRNA