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Protocols



Protocol Title Description File Download SCGE ID
Tsai_CHANGEseq Protocol Procedure for editing quantification using CHANGE-seq. Tsai_CHANGEseq.pdf 21000000005
Tsai_T Cell Transfection Protocol Procedure for T cell transfection. Tsai_T_cell_transfection.pdf 21000000006
Gong_Intracranial Injection Procedure for Mice Procedure for intracranial delivery to mouse brain. Gong_Intracerebral_Injection_Protocol.pdf 21000000020
Deverman_AAV Production and Administration Protocol Published procedures for AAV production, delivery, and tissue imaging. Challis et al. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3. Deverman_AAV_production_and_administration_Nat Protocols-Challis_et_al_2019.pdf 21000000010
Conklin_VectorMOD Protocol Published procedure for mass spectrometry measurement of rAAV capsids. Rumachik et al. VectorMOD: Method for Bottom-Up Proteomic Characterization of rAAV Capsid Post-Translational Modifications and Vector Impurities. Front Immunol. 2021 Apr 1;12:657795. doi: 10.3389/fimmu.2021.657795. eCollection 2021. Conklin_VectorMOD.pdf 21000000018
Heaney-SATC_McCray-Validation_Lung Inflation and Fixation Protocol Procedure for mouse lung inflation and fixation. Heaney-SATC_McCray-Validation_Lung_Inflation_and_Fixation.pdf 21000000022
Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice Protocol Procedure for intranasal instillation of ribonucleoprotein/peptide complex in mice for delivery to the lung. Heaney-SATC_McCray-Validation_Intranasal_Instillation_in_Mice.pdf 21000000021
Murray-SATC_Gong-Validation Brain Injection in Mice Protocol Procedure for brain injection surgical procedure, pre- and post-operative care for mice. Murray-SATC_Gong-Validation_Brain_Injection_in_Mice.pdf 21000000026
Deverman_Comprehensive Methods Procedure for plasmid cloning, editing evaluation in fibroblast, AAV production and administration, tissue processing and IHC. Deverman_Comprehensive_methods.pdf 21000000012
Heaney_SATC Tissue Processing, Imaging and Analysis Procedure for tissue preparation, imaging and analysis. Heaney_SATC_Tissue_Processing_Imaging_and_Analysis.pdf 21000000025
Murray-SATC_Gong-Validaiton_Gong Study Protocol Procedure for intracranial injection, immunofluorescence and imaging. Murray-SATC_Gong-Validation_Gong_ Study_Protocol.pdf 21000000027
Deverman_Area Based Quantification of Editing Efficiency Protocol Procedure for non-IHC based image quantification of editing. Deverman_Area_based_quantification_of_editing_efficiency.pdf 21000000011
Conklin_Fast-Seq AAV Protocol Published procedure for AAV composition measurement using fast-seq. Maynard et al. Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-Associated Viral Genomes in Academic Settings. Hum Gene Ther Methods . 2019 Dec;30(6):195-205. doi: 10.1089/hgtb.2019.110. Conklin_Fast-Seq_AAV.pdf 21000000017