Protocols
This page contains protocols deposited by SCGE consortium laboratories.
| Protocol Title | Description | File Download | SCGE ID |
|---|---|---|---|
| Tsai_CHANGEseq Protocol | Procedure for editing quantification using CHANGE-seq. | Tsai_CHANGEseq.pdf | 21000000005 |
| Tsai_T Cell Transfection Protocol | Procedure for T cell transfection. | Tsai_T_cell_transfection.pdf | 21000000006 |
| Gong_Intracranial Injection Procedure for Mice | Procedure for intracranial delivery to mouse brain. | Gong_Intracerebral_Injection_Protocol.pdf | 21000000020 |
| Deverman_AAV Production and Administration Protocol | Published procedures for AAV production, delivery, and tissue imaging. Challis et al. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3. | Deverman_AAV_production_and_administration_Nat Protocols-Challis_et_al_2019.pdf | 21000000010 |
| Conklin_VectorMOD Protocol | Published procedure for mass spectrometry measurement of rAAV capsids. Rumachik et al. VectorMOD: Method for Bottom-Up Proteomic Characterization of rAAV Capsid Post-Translational Modifications and Vector Impurities. Front Immunol. 2021 Apr 1;12:657795. doi: 10.3389/fimmu.2021.657795. eCollection 2021. | Conklin_VectorMOD.pdf | 21000000018 |
| Heaney-SATC_McCray-Validation_Lung Inflation and Fixation Protocol | Procedure for mouse lung inflation and fixation. | Heaney-SATC_McCray-Validation_Lung_Inflation_and_Fixation.pdf | 21000000022 |
| Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice Protocol | Procedure for intranasal instillation of ribonucleoprotein/peptide complex in mice for delivery to the lung. | Heaney-SATC_McCray-Validation_Intranasal_Instillation_in_Mice.pdf | 21000000021 |
| Murray-SATC_Gong-Validation Brain Injection in Mice Protocol | Procedure for brain injection surgical procedure, pre- and post-operative care for mice. | Murray-SATC_Gong-Validation_Brain_Injection_in_Mice.pdf | 21000000026 |
| Deverman_Comprehensive Methods | Procedure for plasmid cloning, editing evaluation in fibroblast, AAV production and administration, tissue processing and IHC. | Deverman_Comprehensive_methods.pdf | 21000000012 |
| Heaney_SATC Tissue Processing, Imaging and Analysis | Procedure for tissue preparation, imaging and analysis. | Heaney_SATC_Tissue_Processing_Imaging_and_Analysis.pdf | 21000000025 |
| Murray-SATC_Gong-Validaiton_Gong Study Protocol | Procedure for intracranial injection, immunofluorescence and imaging. | Murray-SATC_Gong-Validation_Gong_ Study_Protocol.pdf | 21000000027 |
| Deverman_Area Based Quantification of Editing Efficiency Protocol | Procedure for non-IHC based image quantification of editing. | Deverman_Area_based_quantification_of_editing_efficiency.pdf | 21000000011 |
| Conklin_Fast-Seq AAV Protocol | Published procedure for AAV composition measurement using fast-seq. Maynard et al. Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-Associated Viral Genomes in Academic Settings. Hum Gene Ther Methods . 2019 Dec;30(6):195-205. doi: 10.1089/hgtb.2019.110. | Conklin_Fast-Seq_AAV.pdf | 21000000017 |
| Heaney-SATC_Gao-Validation_Intratracheal Delivery of AAV in Mice | Procedure for Intratracheal (IT) delivery of AAV in mouse lung. | Heaney-SATC_Gao-Validation_Intratracheal_Delivery_of_AAV_in_Mice.pdf | 21000000023 |
| Chaikof-Associated Protocol 1_Retro-orbital administration and High throughput sequencing | This Protocol describes in vivo administration of BE-eVLP for Pcsk9 knockdown in liver, including sequencing endpoint. | Chaikof-Associated Protocol 1_Retro-orbital administration and High throughput sequencing.pdf | 21000000031 |
| Chaikof-Associated Protocol 3_Serum ELISAs | This protocol describes in vivo adminstration and subsequent blood draws and ELISA on C57BL/6J mice. | Chaikof-Associated Protocol 3_Serum ELISAs.pdf | 21000000032 |
| Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis | This protocol describes in vivo adminstration and subsequent analysis of off-target editing in the liver. | Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis.pdf | 21000000033 |
| JAX/UMass SCGE Protocol for mTmG reporter mice | Protocol for intrastriatal injection, dissection and fixation, and immunostaining for GFP expression after activation in mTmG reporter mouse | Jax-Sontheimer SCGE protocol for mTmG reporter mice.pdf | 21000000034 |