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13 results for search term 'Epithelium' in category Protocol

Heaney-SATC_McCray-Validation_Lung Inflation and Fixation Protocol Protocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] Matched Fields: category : Protocol tissueTerm : Epithelium of main bronchus Epithelium of bronchiole termSynonyms : Epithelium of mucosa Columnar epithelium Lung epithelium Epithelium Endo-epithelium Procedure for mouse lung inflation and fixation. Show Experiments (1) Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Heaney-SATC_Gao-Validation_Intratracheal Delivery of AAV in Mice Protocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] Matched Fields: category : Protocol tissueTerm : Epithelium of main bronchus Epithelium of bronchiole termSynonyms : Epithelium of mucosa Columnar epithelium Lung epithelium Epithelium Endo-epithelium Procedure for Intratracheal (IT) delivery of AAV in mouse lung. Show Experiments (1) Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice
Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice Protocol Protocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] Matched Fields: category : Protocol tissueTerm : Epithelium of main bronchus Epithelium of bronchiole termSynonyms : Epithelium of mucosa Columnar epithelium Lung epithelium Epithelium Endo-epithelium Procedure for intranasal instillation of ribonucleoprotein/peptide complex in mice for delivery to the lung. Show Experiments (1) Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Heaney_SATC Tissue Processing, Imaging and Analysis Protocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] Matched Fields: category : Protocol tissueTerm : Epithelium of main bronchus Epithelium of bronchiole termSynonyms : Ecto-epithelium Meso-epithelium Lung epithelium Epithelium Endo-epithelium Procedure for tissue preparation, imaging and analysis. Show Experiments (4) Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Independent validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain
Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis Protocol - [In Vivo] [Delivery Systems Initiative] [Mouse] Matched Fields: category : Protocol termSynonyms : Digestive tract epithelium Columnar epithelium Epithelium Unilaminar epithelium Endo-epithelium This protocol describes in vivo adminstration and subsequent analysis of off-target editing in the liver. Show Experiments (1) On-target editing compared to 14 circle-seq nominated off-target sites of adenine base editor delivered by BE-eVLP vs AAV in the C57BL/6 liver
Chaikof-Associated Protocol 3_Serum ELISAs Protocol - [In Vivo] [Delivery Systems Initiative] [Mouse] Matched Fields: category : Protocol termSynonyms : Columnar epithelium Epithelium Endo-epithelium Meso-epithelium Foregut epithelium This protocol describes in vivo adminstration and subsequent blood draws and ELISA on C57BL/6J mice. Show Experiments (1) Pcsk9 adenine base editor efficiency in liver and nonliver tissue
Chaikof-Associated Protocol 1_Retro-orbital administration and High throughput sequencing Protocol - [In Vivo] [Delivery Systems Initiative] [Mouse] Matched Fields: category : Protocol termSynonyms : Columnar epithelium Epithelium Endo-epithelium Meso-epithelium Foregut epithelium This Protocol describes in vivo administration of BE-eVLP for Pcsk9 knockdown in liver, including sequencing endpoint. Show Experiments (1) Pcsk9 adenine base editor efficiency in liver and nonliver tissue
Deverman_Comprehensive Methods Protocol - [In Vivo, In Vitro] [Delivery Systems Initiative] [Mouse] Matched Fields: category : Protocol termSynonyms : Ecto-epithelium Epithelium Procedure for plasmid cloning, editing evaluation in fibroblast, AAV production and administration, tissue processing and IHC. Show Experiments (2) Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro
JAX/UMass SCGE Protocol for mTmG reporter mice Protocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] Matched Fields: category : Protocol termSynonyms : Ecto-epithelium Epithelium Protocol for intrastriatal injection, dissection and fixation, and immunostaining for GFP expression after activation in mTmG reporter mouse Show Experiments (1) Independent validation of Sontheimer delivery platform using heavily modified guide RNAs complexed with Cas9 proteins to deliver CRISPR/Cas9 to mouse brain
Deverman_Area Based Quantification of Editing Efficiency Protocol Protocol - [In Vivo, In Vitro] [Delivery Systems Initiative] [Mouse] Matched Fields: category : Protocol termSynonyms : Ecto-epithelium Epithelium Procedure for non-IHC based image quantification of editing. Show Experiments (2) Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro
Murray-SATC_Gong-Validation Brain Injection in Mice Protocol Protocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] Matched Fields: category : Protocol termSynonyms : Ecto-epithelium Epithelium Procedure for brain injection surgical procedure, pre- and post-operative care for mice. Show Experiments (1) Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
Murray-SATC_Gong-Validaiton_Gong Study Protocol Protocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] Matched Fields: category : Protocol termSynonyms : Ecto-epithelium Epithelium Procedure for intracranial injection, immunofluorescence and imaging. Show Experiments (1) Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
Gong_Intracranial Injection Procedure for Mice Protocol - [In Vivo] [Delivery Systems Initiative] [Mouse] Matched Fields: category : Protocol termSynonyms : Ecto-epithelium Epithelium Procedure for intracranial delivery to mouse brain. Show Experiments (1) Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.

13 results for search term 'Epithelium' in category Protocol

Name	Description	Source	View Associated..
Heaney-SATC_McCray-Validation_Lung Inflation and Fixation Protocol	Procedure for mouse lung inflation and fixation.		Show Experiments (1) Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Heaney-SATC_Gao-Validation_Intratracheal Delivery of AAV in Mice	Procedure for Intratracheal (IT) delivery of AAV in mouse lung.		Show Experiments (1) Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice
Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice Protocol	Procedure for intranasal instillation of ribonucleoprotein/peptide complex in mice for delivery to the lung.		Show Experiments (1) Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Heaney_SATC Tissue Processing, Imaging and Analysis	Procedure for tissue preparation, imaging and analysis.		Show Experiments (4) Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Independent validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain
Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis	This protocol describes in vivo adminstration and subsequent analysis of off-target editing in the liver.		Show Experiments (1) On-target editing compared to 14 circle-seq nominated off-target sites of adenine base editor delivered by BE-eVLP vs AAV in the C57BL/6 liver
Chaikof-Associated Protocol 3_Serum ELISAs	This protocol describes in vivo adminstration and subsequent blood draws and ELISA on C57BL/6J mice.		Show Experiments (1) Pcsk9 adenine base editor efficiency in liver and nonliver tissue
Chaikof-Associated Protocol 1_Retro-orbital administration and High throughput sequencing	This Protocol describes in vivo administration of BE-eVLP for Pcsk9 knockdown in liver, including sequencing endpoint.		Show Experiments (1) Pcsk9 adenine base editor efficiency in liver and nonliver tissue
Deverman_Comprehensive Methods	Procedure for plasmid cloning, editing evaluation in fibroblast, AAV production and administration, tissue processing and IHC.		Show Experiments (2) Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro
JAX/UMass SCGE Protocol for mTmG reporter mice	Protocol for intrastriatal injection, dissection and fixation, and immunostaining for GFP expression after activation in mTmG reporter mouse		Show Experiments (1) Independent validation of Sontheimer delivery platform using heavily modified guide RNAs complexed with Cas9 proteins to deliver CRISPR/Cas9 to mouse brain
Deverman_Area Based Quantification of Editing Efficiency Protocol	Procedure for non-IHC based image quantification of editing.		Show Experiments (2) Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro
Murray-SATC_Gong-Validation Brain Injection in Mice Protocol	Procedure for brain injection surgical procedure, pre- and post-operative care for mice.		Show Experiments (1) Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
Murray-SATC_Gong-Validaiton_Gong Study Protocol	Procedure for intracranial injection, immunofluorescence and imaging.		Show Experiments (1) Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
Gong_Intracranial Injection Procedure for Mice	Procedure for intracranial delivery to mouse brain.		Show Experiments (1) Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.