SCGE ID11000000104
SymbolCasΦ-1
DescriptionCompact editor of the Cas12j family identified in biggie phage from metagenomic assemblies
SpeciesBiggie Phage
TypeCas Nuclease
SubtypeCas12j
AliasCas12j-1

ActivityDouble strand cleavage
Substratedouble stranded DNA
DSB Cleavage TypeStaggered
PAM5' VTTR
Fusion2X C-terminal SV40NLS, C-terminal 2xFLAG tag

SourceJennifer Doudna Lab

MADTPTLFTQFLRHHLPGQRFRKDILKQAGRILANKGEDATIAFLRGKSEESPPDFQPPVKCPIIACSRPLTEWPIYQA
VAIQGYVYGQSLAEFEASDPGCSKDGLLGWFDKTGVCTDYFSVQGLNLIFQNARKRYIGVQTKVTNRNEKRHKKLKRIN
KRIAEGLPELTSDEPESALDETGHLIDPPGLNTNIYCYQQVSPKPLALSEVNQLPTAYAGYSTSGDDPIQPMVTKDRLS
SKGQPGYIPEHQRALLSQKKHRRMRGYGLKARALLVIVRIQDDWAVIDLRSLLRNAYWRRIVQTKEPSTITKLLKLVTG
PVLDATRMVATFTYKPGIVQVRSAKCLKNKQGSKLFSERYLNETVSVTSIDLGSNNLVAVATYRLVNGNTPELLQRFTL
SHLVKDFERYKQAHDTLEDSIQKTAVASLPQGQQTEIRMWSMYGFREAQERVCQELGLADGSIPWNVMTATSTILTDLF
ARGGDPKKCMFTSEPKKKKNSKQVLYKIRDRAWAKMYRTLLSKETREAWNKALWGLKRGSPDYARLSKRKEELARRCVN
TISTAEKRAQCGRTIVALEDLNIGFFHGRGKQEPGWVGLFTRKKENRWLMQALHKAFLELAHHRGYHVIEVNPAYTSQT
PVCRHCDPDNRDQHNREAFHCIGCGFRGNADLDVATHNIAMVAITGESLKRARGSVASKTPQPLAAEGSGPKKKRKVED
KKKRKVSLGSGSDYKDDDDKDYKDDDDK


Publication Title
CRISPR-CasΦ from huge phages is a hypercompact genome editor. NCBI

This Genome Editor: Casϕ-1 is being used ...
Project Initiative Contact PI
Genome Editors Jennifer A Doudna, PhD Jillian Banfield, PhD
(University of California, Berkeley)
NIH Report