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Filters ..
  Editors
 CasĪ¦-1
 CasĪ¦-2
 CasĪ¦-3
  Guide Target Locus
 eGFP
 non-targeting
  Guides
 Cas12j-GFP1 (CasĪ¦-1)
 Cas12j-GFP1 (CasĪ¦-2)
 Cas12j-GFP10 (CasĪ¦-2)
 Cas12j-GFP10 (CasĪ¦-3)
 Cas12j-GFP11 (CasĪ¦-3)
 Cas12j-GFP12 (CasĪ¦-3)
 Cas12j-GFP13 (CasĪ¦-3)
 Cas12j-GFP14 (CasĪ¦-2)
 Cas12j-GFP14 (CasĪ¦-3)
 Cas12j-GFP15 (CasĪ¦-1)
 Cas12j-GFP16 (CasĪ¦-1)
 Cas12j-GFP17 (CasĪ¦-1)
 Cas12j-GFP18 (CasĪ¦-1)
 Cas12j-GFP19 (CasĪ¦-1)
 Cas12j-GFP2 (CasĪ¦-1)
 Cas12j-GFP2 (CasĪ¦-2)
 Cas12j-GFP20 (CasĪ¦-1)
 Cas12j-GFP21 (CasĪ¦-1)
 Cas12j-GFP22 (CasĪ¦-1)
 Cas12j-GFP23 (CasĪ¦-1)
 Cas12j-GFP3 (CasĪ¦-1)
 Cas12j-GFP3 (CasĪ¦-2)
 Cas12j-GFP4 (CasĪ¦-1)
 Cas12j-GFP4 (CasĪ¦-2)
 Cas12j-GFP5 (CasĪ¦-1)
 Cas12j-GFP5 (CasĪ¦-2)
 Cas12j-GFP5 (CasĪ¦-3)
 Cas12j-GFP6 (CasĪ¦-2)
 Cas12j-GFP6 (CasĪ¦-3)
 Cas12j-GFP7 (CasĪ¦-2)
 Cas12j-GFP7 (CasĪ¦-3)
 Cas12j-GFP8 (CasĪ¦-2)
 Cas12j-GFP8 (CasĪ¦-3)
 Cas12j-GFP9 (CasĪ¦-2)
 Cas12j-GFP9 (CasĪ¦-3)
 SapI-GG stuffer (CasĪ¦-1)
 SapI-GG stuffer (CasĪ¦-2)
 SapI-GG stuffer (CasĪ¦-3)
Delivery Systems
 Lipofectamine 3000
Models
 HEK-293-TgEF1a-eGFP-BSD
Sex
 Female
  Vectors
 pPP394
 pPP441
 pPP444
Select experimental variable to highlight records on the chart: 
 

Note: Hover over the bars to view additional information

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Results

Testing_newly_discovered_Biggie_Phage_editors_in_human_cells
Editing Efficiency
Record Id Condition Sex Editor Model Delivery Target Locus Guide Vector Dosage Editing Efficiency % of GFP negative cells Image
15000000007 Cas12j-GFP1 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP1 (CasĪ¦-1) pPP394 200 ng
15000000008 Cas12j-GFP2 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP2 (CasĪ¦-1) pPP394 200 ng
15000000009 Cas12j-GFP3 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP3 (CasĪ¦-1) pPP394 200 ng
15000000010 Cas12j-GFP4 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP4 (CasĪ¦-1) pPP394 200 ng
15000000011 Cas12j-GFP5 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP5 (CasĪ¦-1) pPP394 200 ng
15000000012 Cas12j-GFP15 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP15 (CasĪ¦-1) pPP394 200 ng
15000000013 Cas12j-GFP16 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP16 (CasĪ¦-1) pPP394 200 ng
15000000014 Cas12j-GFP17 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP17 (CasĪ¦-1) pPP394 200 ng
15000000015 Cas12j-GFP18 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP18 (CasĪ¦-1) pPP394 200 ng
15000000016 Cas12j-GFP19 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP19 (CasĪ¦-1) pPP394 200 ng
15000000017 Cas12j-GFP20 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP20 (CasĪ¦-1) pPP394 200 ng
15000000018 Cas12j-GFP21 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP21 (CasĪ¦-1) pPP394 200 ng
15000000019 Cas12j-GFP22 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP22 (CasĪ¦-1) pPP394 200 ng
15000000020 Cas12j-GFP23 (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP23 (CasĪ¦-1) pPP394 200 ng
15000000021 SapI-GG stuffer (CasĪ¦-1) Female CasĪ¦-1 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 non-targeting SapI-GG stuffer (CasĪ¦-1) pPP394 200 ng
15000000022 Cas12j-GFP1 (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP1 (CasĪ¦-2) pPP441 200 ng
15000000023 Cas12j-GFP2 (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP2 (CasĪ¦-2) pPP441 200 ng
15000000024 Cas12j-GFP3 (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP3 (CasĪ¦-2) pPP441 200 ng
15000000025 Cas12j-GFP4 (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP4 (CasĪ¦-2) pPP441 200 ng
15000000026 Cas12j-GFP5 (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP5 (CasĪ¦-2) pPP441 200 ng
15000000027 Cas12j-GFP6 (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP6 (CasĪ¦-2) pPP441 200 ng
15000000028 Cas12j-GFP7 (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP7 (CasĪ¦-2) pPP441 200 ng
15000000029 Cas12j-GFP8 (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP8 (CasĪ¦-2) pPP441 200 ng
15000000030 Cas12j-GFP9 (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP9 (CasĪ¦-2) pPP441 200 ng
15000000031 Cas12j-GFP10 (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP10 (CasĪ¦-2) pPP441 200 ng
15000000032 Cas12j-GFP14 (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP14 (CasĪ¦-2) pPP441 200 ng
15000000051 SapI-GG stuffer (CasĪ¦-2) Female CasĪ¦-2 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 non-targeting SapI-GG stuffer (CasĪ¦-2) pPP441 200 ng
15000000052 Cas12j-GFP5 (CasĪ¦-3) Female CasĪ¦-3 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP5 (CasĪ¦-3) pPP444 200 ng
15000000053 Cas12j-GFP6 (CasĪ¦-3) Female CasĪ¦-3 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP6 (CasĪ¦-3) pPP444 200 ng
15000000054 Cas12j-GFP7 (CasĪ¦-3) Female CasĪ¦-3 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP7 (CasĪ¦-3) pPP444 200 ng
15000000055 Cas12j-GFP8 (CasĪ¦-3) Female CasĪ¦-3 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP8 (CasĪ¦-3) pPP444 200 ng
15000000056 Cas12j-GFP9 (CasĪ¦-3) Female CasĪ¦-3 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP9 (CasĪ¦-3) pPP444 200 ng
15000000057 Cas12j-GFP10 (CasĪ¦-3) Female CasĪ¦-3 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP10 (CasĪ¦-3) pPP444 200 ng
15000000058 Cas12j-GFP11 (CasĪ¦-3) Female CasĪ¦-3 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP11 (CasĪ¦-3) pPP444 200 ng
15000000059 Cas12j-GFP12 (CasĪ¦-3) Female CasĪ¦-3 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP12 (CasĪ¦-3) pPP444 200 ng
15000000060 Cas12j-GFP13 (CasĪ¦-3) Female CasĪ¦-3 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP13 (CasĪ¦-3) pPP444 200 ng
15000000061 Cas12j-GFP14 (CasĪ¦-3) Female CasĪ¦-3 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 eGFP Cas12j-GFP14 (CasĪ¦-3) pPP444 200 ng
15000000062 SapI-GG stuffer (CasĪ¦-3) Female CasĪ¦-3 HEK-293-TgEF1a-eGFP-BSD Lipofectamine 3000 non-targeting SapI-GG stuffer (CasĪ¦-3) pPP444 200 ng

Publication Title
CRISPR-CasĪ¦ from huge phages is a hypercompact genome editor. NCBI