Testing newly discovered Biggie Phage editors in human cells

Results

Testing_newly_discovered_Biggie_Phage_editors_in_human_cells
									Editing Efficiency
Record Id	Condition	Sex	Editor	Model	Delivery	Target Locus	Guide	Vector	Dosage	Editing Efficiency % of GFP negative cells	Image
15000000007	Cas12j-GFP1 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP1 (CasΦ-1)	pPP394	200 ng	0.97; 0.8099999999999999; 0.9199999999999999;
15000000008	Cas12j-GFP2 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP2 (CasΦ-1)	pPP394	200 ng	1.04; 0.8699999999999999; 0.8699999999999999;
15000000009	Cas12j-GFP3 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP3 (CasΦ-1)	pPP394	200 ng	1.6099999999999999; 0.91; 0.96;
15000000010	Cas12j-GFP4 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP4 (CasΦ-1)	pPP394	200 ng	0.96; 0.76; 1.0;
15000000011	Cas12j-GFP5 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP5 (CasΦ-1)	pPP394	200 ng	0.7799999999999999; 0.8099999999999999; 1.04;
15000000012	Cas12j-GFP15 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP15 (CasΦ-1)	pPP394	200 ng	1.05; 0.9299999999999999; 0.88;
15000000013	Cas12j-GFP16 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP16 (CasΦ-1)	pPP394	200 ng	0.8999999999999999; 0.88; 0.91;
15000000014	Cas12j-GFP17 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP17 (CasΦ-1)	pPP394	200 ng	0.8699999999999999; 1.05; 0.88;
15000000015	Cas12j-GFP18 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP18 (CasΦ-1)	pPP394	200 ng	1.1199999999999999; 0.98; 0.79;
15000000016	Cas12j-GFP19 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP19 (CasΦ-1)	pPP394	200 ng	0.86; 1.0; 0.97;
15000000017	Cas12j-GFP20 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP20 (CasΦ-1)	pPP394	200 ng	0.8999999999999999; 0.9400000000000001; 0.8200000000000001;
15000000018	Cas12j-GFP21 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP21 (CasΦ-1)	pPP394	200 ng	1.13; 0.89; 0.98;
15000000019	Cas12j-GFP22 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP22 (CasΦ-1)	pPP394	200 ng	0.79; 1.0699999999999998; 0.84;
15000000020	Cas12j-GFP23 (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP23 (CasΦ-1)	pPP394	200 ng	1.02; 1.0699999999999998; 0.98;
15000000021	SapI-GG stuffer (CasΦ-1)	Female	CasΦ-1	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	non-targeting	SapI-GG stuffer (CasΦ-1)	pPP394	200 ng	0.9299999999999999; 1.02; 0.79;
15000000022	Cas12j-GFP1 (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP1 (CasΦ-2)	pPP441	200 ng	0.74; 0.88; 0.89;
15000000023	Cas12j-GFP2 (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP2 (CasΦ-2)	pPP441	200 ng	2.19; 1.77; 2.06;
15000000024	Cas12j-GFP3 (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP3 (CasΦ-2)	pPP441	200 ng	3.47; 3.2099999999999995; 3.2;
15000000025	Cas12j-GFP4 (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP4 (CasΦ-2)	pPP441	200 ng	1.1900000000000002; 1.0; 0.88;
15000000026	Cas12j-GFP5 (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP5 (CasΦ-2)	pPP441	200 ng	1.03; 0.6799999999999999; 0.9900000000000001;
15000000027	Cas12j-GFP6 (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP6 (CasΦ-2)	pPP441	200 ng	14.099999999999998; 12.8; 11.799999999999999;
15000000028	Cas12j-GFP7 (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP7 (CasΦ-2)	pPP441	200 ng	1.8800000000000001; 1.6400000000000001; 2.09;
15000000029	Cas12j-GFP8 (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP8 (CasΦ-2)	pPP441	200 ng	32.7; 34.699999999999996; 33.1;
15000000030	Cas12j-GFP9 (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP9 (CasΦ-2)	pPP441	200 ng	23.5; 21.7; 21.2;
15000000031	Cas12j-GFP10 (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP10 (CasΦ-2)	pPP441	200 ng	1.28; 1.0999999999999999; 0.98;
15000000032	Cas12j-GFP14 (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP14 (CasΦ-2)	pPP441	200 ng	2.13; 2.08; 2.2399999999999998;
15000000051	SapI-GG stuffer (CasΦ-2)	Female	CasΦ-2	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	non-targeting	SapI-GG stuffer (CasΦ-2)	pPP441	200 ng	0.79; 0.8200000000000001; 0.8;
15000000052	Cas12j-GFP5 (CasΦ-3)	Female	CasΦ-3	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP5 (CasΦ-3)	pPP444	200 ng	1.5699999999999998; 1.3; 1.66;
15000000053	Cas12j-GFP6 (CasΦ-3)	Female	CasΦ-3	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP6 (CasΦ-3)	pPP444	200 ng	1.6400000000000001; 1.21; 1.27;
15000000054	Cas12j-GFP7 (CasΦ-3)	Female	CasΦ-3	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP7 (CasΦ-3)	pPP444	200 ng	0.8699999999999999; 1.04; 1.06;
15000000055	Cas12j-GFP8 (CasΦ-3)	Female	CasΦ-3	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP8 (CasΦ-3)	pPP444	200 ng	4.1000000000000005; 4.760000000000001; 4.87;
15000000056	Cas12j-GFP9 (CasΦ-3)	Female	CasΦ-3	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP9 (CasΦ-3)	pPP444	200 ng	7.000000000000001; 7.6499999999999995; 7.359999999999999;
15000000057	Cas12j-GFP10 (CasΦ-3)	Female	CasΦ-3	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP10 (CasΦ-3)	pPP444	200 ng	1.8599999999999999; 1.73; 1.71;
15000000058	Cas12j-GFP11 (CasΦ-3)	Female	CasΦ-3	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP11 (CasΦ-3)	pPP444	200 ng	1.0; 1.05; 1.08;
15000000059	Cas12j-GFP12 (CasΦ-3)	Female	CasΦ-3	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP12 (CasΦ-3)	pPP444	200 ng	1.2; 0.79; 0.8999999999999999;
15000000060	Cas12j-GFP13 (CasΦ-3)	Female	CasΦ-3	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP13 (CasΦ-3)	pPP444	200 ng	1.02; 1.17; 0.84;
15000000061	Cas12j-GFP14 (CasΦ-3)	Female	CasΦ-3	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	eGFP	Cas12j-GFP14 (CasΦ-3)	pPP444	200 ng	0.8099999999999999; 0.8200000000000001; 0.75;
15000000062	SapI-GG stuffer (CasΦ-3)	Female	CasΦ-3	HEK-293-TgEF1a-eGFP-BSD	Lipofectamine 3000	non-targeting	SapI-GG stuffer (CasΦ-3)	pPP444	200 ng	0.88; 0.84; 0.89;

Experiment: Testing newly discovered Biggie Phage editors in human cells

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