Contact SCGE




SCGE Consortium Home   |  About SCGE Toolkit   |  Contact Us   |  Citing the Toolkit   |  License  
ExperimentTesting Shuttle Peptides ability to deliver GFP-NLS to airway epithelia.
Experiment ConditionS10, GFP, 1X Delivery
Assay Description: Quantification of GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells.
Tissue Measured:epithelium of main bronchus
Measurement Type:Delivery Efficiency
Record ID15000000280

EditorGFP-NLS
Delivery SystemS10
Delivery System SubtypeAmphiphilic peptide
Guide
Vector

Model SpeciesMouse
Model NameBALB/c

Application Methodnasal instillation
Application Siteairway epithelium
Dosageshuttle peptide (40 µM); GFP-NLS (20 µM)
Injection Frequencyonce
Injection Rate
Injection Volume50 microliters
Days post injection18 hours
Editor Formatribonucleoprotein (RNP)
Antidote Id
Antidote Descriptioncilia (α-tubulin); F-actin (phalloidin stain); nuclei (DAPI)

Measured Values
Samples Size: 4
 
Replicate Result (Delivery Efficiency)
Measurement Units: % of cells
Mean 26.50 
1 20.6 
2 9.0 
3 24.4 
4 52.0 
  

Publication Title
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI