Development System
S10, GFP, 1X Delivery | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia. |
S10, GFP, 1X Delivery |
Quantification of GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells. |
epithelium of main bronchus |
Delivery Efficiency |
15000000280 |
|
GFP-NLS |
S10 |
Amphiphilic peptide |
|
|
|
Mouse |
BALB/c |
|
nasal instillation |
airway epithelium |
shuttle peptide (40 µM); GFP-NLS (20 µM) |
once |
|
50 microliters |
18 hours |
ribonucleoprotein (RNP) |
|
cilia (α-tubulin); F-actin (phalloidin stain); nuclei (DAPI) |
|
Measured Values |
Samples Size: | 4 |
|
Mean |
26.50  |
1 |
20.6  |
2 |
9.0  |
3 |
24.4  |
4 |
52.0  |
| |
|
Publication Title |
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI |