Experiment: Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia.
PI: Paul B McCray, Jr, MD
Description: Delivery of GFP via shuttle peptides to mouse airway epithelium via nasal instilation. Delivery efficiency was quantified in large and small airways by counting the number of GFP positive cells divided by the number of DAPI cells.
Delivery Assays:
Quantification of GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells.
Parent Project: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
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Other experiments in this project: 5
- Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells
- Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
- Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
- Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells.
- Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia
Download: Submitted files
Publications:
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI
Analyze Data Sets Available for this Experiment
Respiratory
| Epithelium of bronchiole |
| Epithelium of main bronchus |
Organ System Overview
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