Experiment Testing virus region 8 (VR8) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice. Experiment Condition AAV9 GFP Assay Description: Relative neuronal transduction levels were quantified by counting the number of transduced neurons, identified based on morphology, for each brain region per 50um sagittal section Tissue Measured: midbrain Cell Type : neuron Measurement Type: Delivery Efficiency Record ID 15000000373 Editor Delivery System Delivery System Subtype Guide Vector AAV9-GFP ; Model Species Mouse Model Name C57BL/6 Application Method intravenous Application Site Dosage 5e13 vg/kg Injection Frequency once Injection Rate Injection Volume 200 microliters Days post injection 4 weeks Editor Format AAV vector encoded Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Delivery Efficiency) Measurement Units: # of cells in 50um Section Mean 99.33  1 41.5  2 145  3 111.5

Representative image of AAV9 GFP delivery in the midbrain. Neuronal and astrocytic expression were visualized by immunohistochemical staining.