Experiment Testing virus region 8 (VR8) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice. Experiment Condition AAVcc81 Assay Description: Relative neuronal transduction levels were quantified by counting the number of transduced neurons, identified based on morphology, for each brain region per 50um sagittal section Tissue Measured: midbrain Cell Type : astrocyte Measurement Type: Delivery Efficiency Record ID 15000000404 Editor Delivery System Delivery System Subtype Guide Vector AAVcc81-GFP; Model Species Mouse Model Name C57BL/6 Application Method intravenous Application Site Dosage 5e13 vg/kg Injection Frequency once Injection Rate Injection Volume 200 microliters Days post injection 4 weeks Editor Format AAV vector encoded Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Delivery Efficiency) Measurement Units: # of cells in 50um Section Mean 17.75  1 14.5  2 21

Representative image of AAVcc81 delivery in the midbrain. Neuronal and astrocytic expression were visualized by immunohistochemical staining.