Experiment Testing virus region 8 (VR8) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice. Experiment Condition AAVcc81 Assay Description: Native fluorescence was quantified using Image J. For each animal, 6 images were taken from 2 sections and used to calculate corrected total cell fluorescence using the formula: Integrated Density – (Area of selected image X Mean fluorescence of background readings). Tissue Measured: heart Measurement Type: Delivery Efficiency Record ID 15000000407 Editor Delivery System Delivery System Subtype Guide Vector AAVcc81-GFP; Model Species Mouse Model Name C57BL/6 Sample Sex Male Application Method intravenous Application Site Dosage 5e13 vg/kg Injection Frequency once Injection Rate Injection Volume 200 microliters Days post injection 4 weeks Editor Format AAV vector encoded Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Delivery Efficiency) Measurement Units: Corrected Total Fluorescence Mean 2654652131.17  1 2682250027.72222  2 2627054234.61111

Representative image of AAVcc81 in heart