Experiment Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Experiment Condition FS66d6 Assay Description: Calculated editing efficiency by % of NGS reads that showed an indel Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000705 Editor SpCas9 (Feldan Therapeutics) Delivery System FS66d6 Delivery System Subtype Amphiphilic peptide Guide McCray_CFTR_Cas9_Guide1 Vector Model Species Human Model Name Primary Airway Epithelia Application Method Application Site Dosage 2uM gRNA, 1.33uM Cas12a, 40uM Shuttle Injection Frequency Injection Rate Injection Volume Days post injection Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 11   Replicate Result (Editing Efficiency) Measurement Units: % of reads Mean 1.79  1 7.77  2 6.28  3 0.24  4 0.8  5 0.44  6 0.6  7 2.37  8 1.16  9 0.0245  10 0.0053  11 0.00485