Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells |
FSD95 |
Calculated editing efficiency by % of NGS reads that showed an indel |
|
Editing Efficiency |
15000000810 |
|
SpCas9 (Feldan Therapeutics) |
FSD95 |
Amphiphilic peptide |
McCray_CFTR_Cas9_Guide1 |
|
|
Human |
Primary Airway Epithelia |
|
|
|
2uM gRNA, 1.33uM Cas12a, 40uM Shuttle |
|
|
|
|
ribonucleoprotein (RNP) |
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|
|
Measured Values |
Samples Size: | 5 |
|
Mean |
3.42  |
1 |
1.65  |
2 |
2.34  |
3 |
3.24000000953674  |
4 |
6.27500009536743  |
5 |
3.61000001430511  |
| |
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