NC-No Ligand delivery of CRISPR RNP produces neuronal genome editing in animal UW2
Representative coronal brain images from an Ai14 reporter mouse intrastriatally injected with not decorated nanocages (NC-no ligand) carrying CRISPR Cas9/sgRNA ribonucleoprotein. The brain sections are shown in rostrocaudal (top to bottom) anatomical order. The colors correspond to tdTomato-expressing, genome-edited cells (red; white arrows pointing to edited area) with NeuN+ neurons (green), GFAP+ astrocytes (pink), and DAPI labelled nuclei (blue).
NC-No Ligand delivery of CRISPR RNP produces neuronal genome editing in animal UW3
Representative coronal brain images from an Ai14 reporter mouse intrastriatally injected with not decorated nanocages (NC-no ligand) carrying CRISPR Cas9/sgRNA ribonucleoprotein. The brain sections are shown in rostrocaudal (top to bottom) anatomical order. The colors correspond to tdTomato-expressing, genome-edited cells (red; white arrows pointing to edited area) with NeuN+ neurons (green), GFAP+ astrocytes (pink), and DAPI labelled nuclei (blue).
