sNLS-SpCas9-sNLS | SCGE Toolkit

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SCGE ID	11000000133
Symbol	sNLS-SpCas9-sNLS
Description	SpCas9 with N- and C-terminal SV40 NLS
Species	Streptococcus pyogenes
Type	Cas Nuclease
Subtype	Cas9

Activity	Double strand cleavage
Substrate	double stranded DNA
DSB Cleavage Type	Blunt
PAM	3' NGG
Fusion	N- and C-terminal SV40 NLS

Source	Aldevron 9212-5MG

AAGTAAAACCTCTACAAATG

This Genome Editor: sNLS-SpCas9-sNLS is being used ...

Project	Initiative	Contact PI
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Experiments Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.	Delivery Systems	Shaoqin (Sarah) Gong, PhD (University of Wisconsin-Madison)
The Jackson Laboratory Gene Editing Testing Center (JAX-GETC) Experiments Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain	Animal Reporter and Testing Center	Stephen A Murray, PhD (Jackson Laboratory)
Comparing the efficiency and cellular impact of non-viral, hepatocyte-targeted delivery vehicles with viral-mediated gene delivery in a microphysiological liver on chip platform Experiments Testing different ratios of Lipofectamine-RNPs after 24 hours for determination of positive control Quantification of transfection efficiency by flow cytometry with varying dosages of nanoparticles encapsulated with Cas9/sgRNA RNP on liver-on-chip model system Imaging quantification of transfection efficiency with varying dosages of nanoparticles encapsulated with Cas9/sgRNA RNP on the liver-on-chip model system	Collaborative Opportunity Fund	Ross C. Wilson, PhD Samira Kiani, PhD Shaoqin (Sarah) Gong, PhD (University of Pittsburgh)