Ai14 gRNA | SCGE Toolkit

SCGE ID	10000000244
Lab ID	Ai14 gRNA
Source	IDT
Target Locus	Ai9/Ai14 reporter transgene
Species	transgene
Description	This sgRNA targets the Ai9 and related transgenes at multiple sites
Guide Compatibility	SpyCas9

Target Sequence	AAGTAAAACCTCTACAAATG
Target Sequence + PAM	AAGTAAAACCTCTACAAATGTGG

Spacer Sequence	AAGUAAAACCUCUACAAAUG
Spacer Length	20

This Guide: Ai14 gRNA is being used ...

Project	Initiative	Contact PI
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Experiments Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.	Delivery Systems	Shaoqin (Sarah) Gong, PhD (University of Wisconsin-Madison)
The Jackson Laboratory Gene Editing Testing Center (JAX-GETC) Experiments Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain	Animal Reporter and Testing Center	Stephen A Murray, PhD (Jackson Laboratory)

Guide: Ai14 gRNA