Experiment: Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
PI: Shaoqin (Sarah) Gong, PhD>
Description: Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression.
Editing Assay:
Editing efficiency calculated by counting the percent of NeuN+ neurons expressing tdTomato surrounding the injected area of the striatum
Parent Project: Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
Download: Submitted files
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Results |
| Editing Efficiency | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Condition | Tissue | Cell Type | Sex | Editor | Model | Delivery | Target Locus | Guide | Dosage | Injection Frequency | % of cells | Image |
| RNP-NC-CPP | cerebral cortex (TARGET) | neuron | Male/Female | sNLS-SpCas9-sNLS | Ai14 mouse | RNP-NC-CPP | Ai9/Ai14 reporter transgene | Ai14 gRNA | 30 pmol | once bilaterally |
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| RNP-NC-no ligand | cerebral cortex (TARGET) | neuron | Female | sNLS-SpCas9-sNLS | Ai14 mouse | RNP-NC-no ligand | Ai9/Ai14 reporter transgene | Ai14 gRNA | 30 pmol | once bilaterally |
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Experiment Wide Protocols |
| Title | Description | File Download | SCGE ID |
|---|---|---|---|
| Gong_Intracranial Injection Procedure for Mice | Procedure for intracranial delivery to mouse brain. | Gong_Intracerebral_Injection_Protocol.pdf | 21000000020 |
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