Development System
S10 | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
| Gene editing in vitro by various peptide variants delivering Cas12a in NK cells. |
| S10 |
| Calculated editing efficiency by T7E1 digestion. |
|
| Editing Efficiency |
| 15000000531 |
|
| AsCas12a (Feldan Therapeutics) |
| S10 |
| Amphiphilic peptide |
| NKG2A |
|
|
| Human |
| NK |
|
|
|
| 1.33uM Cas12a/2.0uM gRNA/7.5uM Shuttle Peptide |
|
|
|
| 48 hours |
| ribonucleoprotein (RNP) |
|
|
|
| Measured Values |
| Samples Size: | 3 |
| |
| Mean |
24.99  |
| 1 |
23.95680  |
| 2 |
24.02615  |
| 3 |
26.98109  |
| |
|
| Publication Title |
|
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI |