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ExperimentGene editing in vitro by various peptide variants delivering Cas12a in NK cells.
Experiment ConditionCM18-PTD4
Assay Description: Calculated editing efficiency by T7E1 digestion.
Tissue Measured:
Measurement Type:Editing Efficiency
Record ID15000000534

EditorAsCas12a (Feldan Therapeutics)
Delivery SystemCM18-PTD4
Delivery System SubtypeAmphiphilic peptide
GuideNKG2A
Vector

Model SpeciesHuman
Model NameNK

Application Method
Application Site
Dosage1.33uM Cas12a/2.0uM gRNA/7.5uM Shuttle Peptide
Injection Frequency
Injection Rate
Injection Volume
Days post injection48 hours
Editor Formatribonucleoprotein (RNP)
Antidote Id
Antidote Description

Measured Values
Samples Size: 3
 
Replicate Result (Editing Efficiency)
Measurement Units: % indel
Mean 9.83 
1 8.66051 
2 8.90658 
3 11.92783 
  

Publication Title
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI