Development System
CM18-PTD4, Cas9, CFTR | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. |
CM18-PTD4, Cas9, CFTR |
Calculated editing efficiency mean of 3 biological replicates by sanger sequencing. |
|
Editing Efficiency |
15000000546 |
|
SpCas9 (Feldan Therapeutics) |
CM18-PTD4 |
Amphiphilic peptide |
g-11_C9 |
|
|
Human |
Primary Airway Epithelia |
|
In Media |
|
[RNP]: 2.5 µM; [gRNA]: 2.0 μM; [Peptide]: 40uM |
|
|
|
72 hours |
ribonucleoprotein (RNP) |
|
|
|
Measured Values |
Samples Size: | 1 |
|
Mean |
11.00  |
1 |
11.0  |
| |
|
Publication Title |
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI |