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ExperimentGene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells.
Experiment ConditionCM18-PTD4, Cas9, CFTR
Assay Description: Calculated editing efficiency mean of 3 biological replicates by sanger sequencing.
Tissue Measured:
Measurement Type:Editing Efficiency
Record ID15000000546

EditorSpCas9 (Feldan Therapeutics)
Delivery SystemCM18-PTD4
Delivery System SubtypeAmphiphilic peptide
Guideg-11_C9
Vector

Model SpeciesHuman
Model NamePrimary Airway Epithelia

Application MethodIn Media
Application Site
Dosage[RNP]: 2.5 µM; [gRNA]: 2.0 μM; [Peptide]: 40uM
Injection Frequency
Injection Rate
Injection Volume
Days post injection72 hours
Editor Formatribonucleoprotein (RNP)
Antidote Id
Antidote Description

Measured Values
Samples Size: 1
 
Replicate Result (Editing Efficiency)
Measurement Units: % indel
Mean 11.00 
1 11.0 
  

Publication Title
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI