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Experiment: Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells

PI:   Erik J Sontheimer, PhD 
 
 

Chemically modified crRNA and tracrRNA were delivered by electroporation to transgenic human HEK-293T cells harboring the TLR1 reporter. Gene editing was determined by reporter activation.
Editing Assay: - Calculated editing efficiency by flow cytometry analysis of RFP.

Filter Results ..
Editors
 SpyCas9-3xNLS
Guide Target Locus
 TLR1 reporter
Guides
 STS120 (TLR1; Sp_t2:Sp_c20_TLR1)
Models
 HEK-293T-disrupted_GFP-mcherry-Puro
Sex
 Female

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Results

Testing_newly_chemically_modified_crRNA_and_tracrRNA_to_activate_the_TLR1_reporter_in_human_cells
Editing Efficiency
Record Id Condition Sex Editor Model Target Locus Guide Dosage Editing Efficiency % of RFP+ cells Image
15000000552 STS120 (TLR1) (3 pmol RNP) Female SpyCas9-3xNLS HEK-293T-disrupted_GFP-mcherry-Puro TLR1 reporter STS120 (TLR1; Sp_t2:Sp_c20_TLR1) 3 pmol RNP
15000000553 STS120 (TLR1) (20 pmol RNP) Female SpyCas9-3xNLS HEK-293T-disrupted_GFP-mcherry-Puro TLR1 reporter STS120 (TLR1; Sp_t2:Sp_c20_TLR1) 20 pmol RNP