Development System Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells | SCGE Toolkit Chemically modified crRNA and tracrRNA were delivered by electroporation to transgenic human HEK-293T cells harboring the TLR1 reporter. Gene editing was determined by reporter activation.
Experiment: Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells
PI:
Erik J Sontheimer, PhD
Chemically modified crRNA and tracrRNA were delivered by electroporation to transgenic human HEK-293T cells harboring the TLR1 reporter. Gene editing was determined by reporter activation.
Editing Assay:
- Calculated editing efficiency by flow cytometry analysis of RFP.
Make a selection to highlight records on the chart:
Results
SCGE Toolkit downloaded on: 2023/03/22 11:19:37; Please cite the Somatic Cell Genome Editing Consortium Toolkit NIH HG010423 when using publicly accessible data in formal presentation or publication. SCGE Experment ID: 18000000019. PI:
Erik J Sontheimer PhD
