Experiment Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells Experiment Condition STS135 (PCSK9c) (20 pmol RNP) Assay Description: Calculated editing efficiency by PCR amplification and sanger sequencing analysis. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000557 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS135 (PCSK9c; Sp_t2:Sp_c20_PCSK9c) Vector Model Species Mouse Model Name Hepa1-6 Application Method electroporation Application Site Dosage 20 pmol RNP Injection Frequency Injection Rate Injection Volume Days post injection 72 hours Editor Format protein, RNA Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: % of Lesion Mean 88.5  1 93  2 84