Experiment Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells Experiment Condition STS204 (DNMT1) (5 pmol RNP) Assay Description: Calculated editing efficiency by PCR amplification and sanger sequencing analysis. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000560 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS204 (DNMT1; Sp_t41:Sp_c20_Dnmt1) Vector Model Species Mouse Model Name Neuro 2A Application Method electroporation Application Site Dosage 5 pmol RNP Injection Frequency Injection Rate Injection Volume Days post injection 72 hours Editor Format protein, RNA Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: % of Lesion Mean 24.1  1 19.4  2 28.8