Delivery of Cas9/sgRNA mixed with 306-S10 into stria vascularis adult mouse cochlea via cochleostomy
Delivery of Cas9/sgRNA mixed with 306-S10 into stria vascularis adult mouse cochlea via cochleostomy. a-f. Whole mount immunofluorescence staining of the base region of the cochlea using GJB2 (basal cell marker, green) and ATP1A1 (marginal cell marker, cyan). Cochlea tissues were collected 3-6 days post injection. tdT signals were observed in stria region, presumably in intermediate cells and marginal cells. (a-b), low magnification images; (c-d), high magnification images. (e-f) cross section view of (c-d). G, illustration of cells types with tdT signal in cochlea. H, quantification of tdT+ cells in cochlea. N=3, mean +/- SEM. DC, Deiters cells; OPC, outer pillar cells; IHC, inner hair cells.