Ai14 mouse (congenic) | SCGE Toolkit

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Summary Projects & Experiments

SCGE ID	13000000031
Name	Ai14 mouse (congenic)
Official Name	B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J
Species	Mouse
Type	Animal
Description	Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.

Source	The Jackson Laboratory
RRID	RRID:IMSR_JAX:007914

Transgene	Ai9 (CAG-lox-stop-lox-tdTomato)
Transgene Description	CAG-loxP-stop-loxP-tdtomato reporter cassette which is activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair
Reporter	tdTomato
Annotated Map	addgene-plasmid-22799-sequence-115994.gbk

Publication Title
A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. NCBI
Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice. NCBI

This Model System: Ai14 mouse (congenic) is being used ...

Project	Initiative	Contact PI
Efficient In Vivo RNP-Based Gene Editing in the Sensory Organ Inner Ear Using Bioreducible Lipid Nanoparticles Experiments Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea	Delivery Systems	Zheng-Yi Chen, PhD (Massachusetts Eye & Ear Infirmary)
The Jackson Laboratory Gene Editing Testing Center (JAX-GETC) Experiments Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain	Animal Reporter and Testing Center	Stephen A Murray, PhD (Jackson Laboratory)