Experiment: Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
PI: Zheng-Yi Chen, PhD
Description: Delivery of Cas9/sgRNA mRNA via new LNPs to the cochlea by cochleostomy and gene editing is measured by percentage of tdTomato positive cells.
Editing Assay:
Editing efficiency is calculated by percentage of tdTomato positive cells
Parent Project: Efficient In Vivo RNP-Based Gene Editing in the Sensory Organ Inner Ear Using Bioreducible Lipid Nanoparticles
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Other experiments in this project: 3
- Verification of new LNP 306-O12B for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea. Procedure performed side by side with JAX.
- Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea
- Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice
Download: Submitted files
Analyze Data Sets Available for this Experiment
Sensory
| Base of cochlear canal (Deiter's cell) |
| Scala tympani (Deiter's cell) |
| Base of cochlear canal (cochlear inner hair cell) |
| Base of cochlear canal (external pillar cell of cochlea) |
| Scala tympani (cochlear inner hair cell) |
| Stria vascularis of cochlear duct |
Organ System Overview
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