Experiment: Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
PI: Zheng-Yi Chen, PhD
Description: Delivery of Cas9/sgRNA mRNA via new LNPs to the cochlea by cochleostomy and gene editing is measured by percentage of tdTomato positive cells.
Editing Assay:
Editing efficiency is calculated by percentage of tdTomato positive cells
Parent Project: Efficient In Vivo RNP-Based Gene Editing in the Sensory Organ Inner Ear Using Bioreducible Lipid Nanoparticles
-
Other experiments in this project: 3
- Verification of new LNP 306-O12B for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea. Procedure performed side by side with JAX.
- Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea
- Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice
Download: Submitted files
Validation:
- Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
- Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
- Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
- Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Select experimental variable to highlight records on the chart:
Note: Hover over the bars to view additional information
Results |
| Editing Efficiency | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Condition | Tissue | Cell Type | Editor | Model | Delivery | Target Locus | Guide | Dosage | Injection Frequency | % of cells | Image |
| 306-O12B | base of cochlear canal | Deiter's cell | CleanCap® Cas9 | Ai14 mouse (congenic) | 306-O12B | Ai9/Ai14 reporter transgene | sg298 | LNP/Cas9/sgRNA=0.15/0.01/0.01ug | once |
 |
|
| 306-O12B | scala tympani | Deiter's cell | CleanCap® Cas9 | Ai14 mouse (congenic) | 306-O12B | Ai9/Ai14 reporter transgene | sg298 | LNP/Cas9/sgRNA=0.15/0.01/0.01ug | once |
 |
|
| 113-O12B | base of cochlear canal | Deiter's cell | CleanCap® Cas9 | Ai14 mouse (congenic) | 113-O12B | Ai9/Ai14 reporter transgene | sg298 | LNP/Cas9/sgRNA=0.15/0.01/0.01ug | once |
 |
|
| 113-O12B | base of cochlear canal | cochlear inner hair cell | CleanCap® Cas9 | Ai14 mouse (congenic) | 113-O12B | Ai9/Ai14 reporter transgene | sg298 | LNP/Cas9/sgRNA=0.15/0.01/0.01ug | once |
 |
|
| 306-S10 | base of cochlear canal | Deiter's cell | CleanCap® Cas9 | Ai14 mouse (congenic) | 306-S10 | Ai9/Ai14 reporter transgene | sg298 | LNP/Cas9/sgRNA=0.15/0.01/0.01ug | once |
 |
|
| 306-S10 | scala tympani | Deiter's cell | CleanCap® Cas9 | Ai14 mouse (congenic) | 306-S10 | Ai9/Ai14 reporter transgene | sg298 | LNP/Cas9/sgRNA=0.15/0.01/0.01ug | once |
 |
|
| 306-S10 | base of cochlear canal | external pillar cell of cochlea | CleanCap® Cas9 | Ai14 mouse (congenic) | 306-S10 | Ai9/Ai14 reporter transgene | sg298 | LNP/Cas9/sgRNA=0.15/0.01/0.01ug | once |
 |
|
| 306-S10 | scala tympani | cochlear inner hair cell | CleanCap® Cas9 | Ai14 mouse (congenic) | 306-S10 | Ai9/Ai14 reporter transgene | sg298 | LNP/Cas9/sgRNA=0.15/0.01/0.01ug | once |
 |
|
| 306-S10 | stria vascularis of cochlear duct | CleanCap® Cas9 | Ai14 mouse (congenic) | 306-S10 | Ai9/Ai14 reporter transgene | sg298 | LNP/Cas9/sgRNA=0.15/0.01/0.01ug | once |
 |
||
× Close Overview
