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ExperimentDelivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
Experiment ConditionSp_c20_mTmG/Sp_t2
Assay Description: Editing efficiency determined by immunohistochemical staining for GFP.
Tissue Measured:brain
Measurement Type:Editing Efficiency
Record ID15000000701

EditorSpyCas9-3xNLS
Delivery System
Delivery System Subtype
GuideSTS159 (mTmG; Sp_t2:Sp_c20_mTmG)
Vector

Model SpeciesMouse
Model NameB6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J

Application MethodIntra-Striatum
Application Sitebrain
Dosage100 µM RNP
Injection Frequencyonce
Injection Rate500 nl/min
Injection Volume2-3 microliters
Days post injection1 or 2 weeks
Editor Formatribonucleoprotein (RNP)
Antidote Id
Antidote Description

Antibody RRIDRRID:AB_2536526
Antibody Other IDThermo Fisher Scientific Cat# G10362
Antibody DescriptionGFP Recombinant Rabbit Monoclonal Antibody, Thermo Fisher Scientific #G10362

Measured Values
Samples Size: 4
 
Replicate Result (Editing Efficiency)
Measurement Units: Signal
Mean 4 of 4 present 
1 present 
2 present 
3 present 
4 present