Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain |
Sp_c20_mTmG/Sp_t2 |
Editing efficiency determined by immunohistochemical staining for GFP. |
brain |
Editing Efficiency |
15000000701 |
|
SpyCas9-3xNLS |
|
|
STS159 (mTmG; Sp_t2:Sp_c20_mTmG) |
|
|
Mouse |
B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J |
|
Intra-Striatum |
brain |
100 µM RNP |
once |
500 nl/min |
2-3 microliters |
1 or 2 weeks |
ribonucleoprotein (RNP) |
|
|
|
RRID:AB_2536526 |
Thermo Fisher Scientific Cat# G10362 |
GFP Recombinant Rabbit Monoclonal Antibody, Thermo Fisher Scientific #G10362 |
|
Measured Values |
Samples Size: | 4 |
|
Mean |
4 of 4 present  |
1 |
present  |
2 |
present  |
3 |
present  |
4 |
present  |